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Biotecnologia Aplicada
Elfos Scientiae
ISSN: 0684-4551
Vol. 12, No. 1, 1995, pp. 36-41

Bioline Code: ba95006
Full paper language: English
Document type: Short Communication
Document available free of charge

Biotecnologia Aplicada, Vol. 12, No. 1, 1995, pp. 36-41

 en Human blood group B trisaccharide: Synthesis, characterization and use in the generation and selection of monoclonal antibodies with a known specificity
Bencomo, Antonio; Campos, Maria T.; Alaez, Carlos; Bencomo, Vicente Verez; Sarracent, Jorge; Rodriguez, Juan C. & Herrera, Mario

Abstract

The trisaccharide specific for human blood group B was obtained as a glycoside of 8-hydroxy-3,6-dioxaoctanal. A new galactose intermediate was developed for chain extension at 0-2 or 0-3 in either sequence. The use of trichloroacetimidates as glycosyl donors for the establishment of the two a-glycosidic linkages was also noteworthy. Human blood group B trisaccharide coupled to KLH was used to induce high anti-B titer in balb-c mice for the production of anti-B monoclonal antibodies. The hybridomas were selected by their reaction with the trisaccharide and by their specific agglutination of B erythrocytes. The monoclonal antibody LAGS-B-03 thus selected displayed excellent parameters as a blood-typing reagent.

Keywords
Trisaccharide, ELISA, synthesis

 
 es
Bencomo, Antonio; Campos, Maria T.; Alaez, Carlos; Bencomo, Vicente Verez; Sarracent, Jorge; Rodriguez, Juan C. & Herrera, Mario

Resumen

Un trisacarido especifico del grupo sanguineo humano B fue obtenido en forma de glicosido del 8-hidroxi-3,6-dioxaoctanal. La sintesis contempla el uso del metodo de los tricloroacetimidatos para establecer los dos enlaces a y un nuevo intermediario de la galactosa que puede ser extendido indistintamente por las posiciones 2 y 3. El trisacarido del grupo sanguineo humano B acoplado a KLH se empleo para inducir alto titulo anti-B en ratones balb-c para la produccion de anticuerpos monoclonales anti-B. Los hibridomas fueron seleccionados sobre la base de su reaccion con el trisacarido y luego por su capacidad de aglutinar especificamente los eritrocitos B. El anticuerpo LAGS-B-03 seleccionado demostro excelentes parametros como reactivo hemoclasificador.

 
© Copyright 1995 Sociedad Iberolatinoamericana de Biotecnologia Aplicada a la Salud
Alternative site location: http://elfosscientiae.cigb.edu.cu/Archivo.asp?Id=6

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