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Journal of Culture Collections
National Bank for Industrial Microorganisms and Cell Cultures
ISSN: 1310-8360
Vol. 5, No. 1, 2006, pp. 58-63
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Bioline Code: cc06008
Full paper language: English
Document type: Research Article
Document available free of charge
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Journal of Culture Collections, Vol. 5, No. 1, 2006, pp. 58-63
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ISOLATION OF BACTERIOCINOGENIC LACTIC ACID BACTERIA FROM RAT INTESTINE
Patil, Mahantesh; Pal, Ajay; Pal, Vijai & Yaddula, Rajini Kumari
Abstract
Lactic acid bacteria were isolated from rat intestinal contents and screened for bacteriocin production. Out of twenty-three isolates, eight were found to produce bacteriocins. The morphological and biochemical properties of these isolates indicated that they belong to the genus Enterococcus. The bacteriocins were extracted and partially purified by Gel Permeation Chromatography (GPC) using G-25, and the antimicrobial spectrum of these bacteriocins was studied against indicator organisms, including Lactococcus diacetylactis, Staphylococcus aureus, Escherichia coli and Pseudomonas sp.
Keywords
lactic acid bacteria, bacteriocins, Enterococcus, intestine.
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