Actinomycetes, 1991, Vol.2, Part 2. pp.48-50.
Ribosomal protein patterns in two-dimensional PAGE as a new
approach for identification and classification of
Code Number: AC91010
Size of Files:
Graphics: photographs (jpg) - 58K
Bacterial ribosomes consist of three species (5S, 16S, and
23S) of RNA and more than 50 species of ribosomal proteins
(for reviews, see references(1) and(2)). Because of many
structural and functional constraints, the variability of
ribosomal proteins (and also rRNA) is more limited than the
variability of other proteins; thus, ribosomal proteins have
much lower evolutionary rates than other proteins(3).
Therefore, analysis of ribosomal proteins and rRNAs could be
an excellent approach for studying the evolutionary
relationships among organisms. Although ribosomal proteins and
rRNA have been studied in great detail in Escherichia coli,
only few reports are available on the ribosomal proteins
of actinomycetes(4,5). The use of two-dimensional separation
of ribosomal proteins for identification and classification
has been extended to the family Enterobacteriaceae,
the family Bacillaceae, and several other bacteria(6-8).
Copyright 1996 C.E.T.A., The International Centre for
Theoretical and Applied Ecology, Gorizia