Actinomycetes, Vol. 4, Part 3, 59-64, 1993
Strategy for the use of pretreatments in the isolation of
non-streptomycete actinomycetes from soil
P. F. LONG and H. G. WILDMAN
Code Number: AC93008
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ABSTRACT. A strategy for the isolation of a diverse range of
non-streptomycete actinomycetes is proposed using chemical
pretreatments before depletion of streptomycete numbers using
bacteriophage. The two pretreatments, 0.05% sodium dodecyl
sulphate (SDS) and O.1M tetrasodium pyrophosphate
(Na[4]P0[2]0[7]), had differing effects on the total
actinomycetes recovered. The physicochemical characteristics
of the soil may have been responsible for this. Although the
number of non-streptomycetes isolated was not significantly
altered by each pretreatment, the use of the pretreatments
significantly changed the diversity of the non-streptomycete
isolates in some soils. The use of the two chemicals is
therefore recommended in addition to air drying soil at room
temperature when isolating non-streptomycete actinomycetes
from soil.
Copyright 1993 C.E.T.A.
