Actinomycetes, Volume VI, Number 1, 1995
PARTIAL CHARACTERIZATION OF STREPTOMYCES PHAGES
ISOLATED FROM THE SOILS OF JARRAH FOREST IN WESTERN
AUSTRALIA
K.A. EL-TARABILY, D.I. KURTBOKE and G.E.ST.J. HARDY
Code Number: AC95002
Size of Files:
Text: 29K
Graphics: Photos (jpg) - 60.7K / Halftones (gif) - 83K
NOTE: Strains designated f below are designated theta in the
printed edition.
ABSTRACT.
Three phages fS1, fS2 and fS3 which utilized Streptomyces
diastaticus, S.griseus and S.hygroscopicus respectively, as
propagation hosts, were isolated from the soils of jarrah
forest in Western Australia. They were partially characterized
through their physiochemical properties, plaque morphology,
host range and particle morphology. Host range of these three
Siphoviridae (B1) morphotype phages demonstrated a wide
activity spectra within the genus Streptomyces. Adsorption
rate constants and burst sizes of the three phages were in the
range of 1.58x10-7, 1.26x10-7 and 5.97x10-9 ml/min and 15.5,
22.5, 12.9 virions/cell, respectively. Agarose electrophoresis
of restriction endonuclease digests of the phage DNAs
indicated that the three phages were different.
Copyright 1995 CECT
