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Actinomycetes
University of Udine, Mycology Department
ISSN: 0732-0574
Vol. 8, No. 3, 1997
Bioline Code: ac97010
Full paper language: English
Document type: Research Article
Document available free of charge

Actinomycetes, Vol. 8, No. 3, 1997

 en Detection of giant linear plasmids in off-flavor compound-producing strains of Streptomyces by PFGE

Abstract

Actinomycetes, 1997, Vol.8, Part 3. pp.58-65 

Detection of giant linear plasmids in off-flavor compound-producing strains
of Streptomyces by PFGE

I. SAADOUN and W.T. BLEVINS

Code Number: AC97010
Sizes of Files:
    Text: 21.2K
    Graphics: Photographs (jpg) - 100.2K

ABSTRACT. 

A geosmin-producing actinomycete, identified as Streptomyces halstedii and
isolated from a catfish production pond, and a MIB-producer, isolated from
a drinking water reservoir and identified as S. violaceusniger, were used
to investigate the presence of plasmids. Several lytic techniques were
compared for recovery of chromosomnal and plasmid DNA. Using an
alkaline-lysis DNA extraction procedure and conventional gel
electrophoresis, no low molecular weight plasmids were observed in any of
the isolates tested. However, lysis of Streptomyces cells in low melting
point agarose followed by pulsed-field electrophoresis (CHEF-DR II) of the
DNA yielded high-molecular weight plasmids.

Copyright 1997  C.E.T.A., The International Centre for Theoretical and
Applied Ecology, Gorizia

 

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