Actinomycetes, 1997, Vol.8, Part 3. pp.58-65
Detection of giant linear plasmids in off-flavor compound-producing strains
of Streptomyces by PFGE
I. SAADOUN and W.T. BLEVINS
Code Number: AC97010
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ABSTRACT.
A geosmin-producing actinomycete, identified as Streptomyces halstedii and
isolated from a catfish production pond, and a MIB-producer, isolated from
a drinking water reservoir and identified as S. violaceusniger, were used
to investigate the presence of plasmids. Several lytic techniques were
compared for recovery of chromosomnal and plasmid DNA. Using an
alkaline-lysis DNA extraction procedure and conventional gel
electrophoresis, no low molecular weight plasmids were observed in any of
the isolates tested. However, lysis of Streptomyces cells in low melting
point agarose followed by pulsed-field electrophoresis (CHEF-DR II) of the
DNA yielded high-molecular weight plasmids.
Copyright 1997 C.E.T.A., The International Centre for Theoretical and
Applied Ecology, Gorizia