Actinomycetes, Vol. 9, Part 3, 1998, pp.46-51
Evaluation of different methods of plasmid extraction from
antibiotic-producing strains of Streptomyces
I. Saadoun, F. Al-Momani and A. Elbetieha
Code Number:AC98007
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ABSTRACT.
Members of the genus Streptomyces isolated from
nature carry detectable covalently closed circular (CCC)-DNA. Four
different extraction methods of small plasmid DNA from
antibiotic-producing Streptomyces isolates and from the
positive control S. lividans ATCC 35287, containing
the pIJ702 plasmid, are reported. Only one procedure allowed
detection of a plasmid from the positive control. No low molecular
weight plasmids were observed in Streptomyces strains No.
264 and No. 404 suggesting that antibiotic production in these
strains is likely to be chromosomally encoded.
Copyright 1998 C.E.T.A., The International Centre for Theoretical
and Applied Ecology, Gorizia
