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Agricultura Técnica
Instituto de Investigaciones Agropecuarias, INIA
ISSN: 0365-2807
EISSN: 0365-2807
Vol. 64, No. 1, 2004, pp. 17-24
Bioline Code: at04002
Full paper language: Spanish
Document type: Research Article
Document available free of charge

Agricultura Técnica, Vol. 64, No. 1, 2004, pp. 17-24

 es Evaluación de dos Cepas del Hongo Metarhizium Anisopliae var. Anisopliae (Metsh.) para el Control de Larvas de Gusano Blanco Hylamorpha Elegans Burm. (Coleoptera: Scarabaeidae)
Rodríguez, Marta; France, Andrés & Gerding, Macarena

Resumen

El gusano blanco, Hylamorpha elegans Burm., es una plaga grave en cereales y praderas en Chile. Se evaluó en laboratorio e invernadero la patogenicidad de dos aislamientos del hongo Metarhizium anisopliae var. anisopliae: Qu-M270 y Qu-M802, sobre larvas de tercer estadio de Hylamorpha elegans Burm. La primera evaluación se realizó por inmersión de las larvas en suspensiones crecientes de 0 a 108 conidias mL-1 de cada aislamiento. Se calculó el área bajo la curva de la mortalidad acumulada, observándose diferencias (P = 0,032) entre aislamientos, siendo Qu-M270 superior en un 50% a Qu-M802. Las CL50 y CL90 para Qu-M270 fueron 104,7 y 107,5 conidias mL-1, respectivamente. Las evaluaciones en invernadero se realizaron en macetas con suelo pasteurizado y 10 larvas L3 de H. elegans, sobre las cuales se pulverizaron cuatro suspensiones (0 a 1014 conidias ha-1). Luego de 30 días, no hubo diferencias significativas en la mortalidad (P < 0,05) entre dosis de 1013 y 1014 conidias ha-1, con 95 y 94% de mortalidad, respectivamente, pero sí respecto a 1012 conidias ha-1 (P = 0,045), la que fue de 74%. Se concluyó que Qu-M270 fue más efectiva en el control de larvas de H. elegans.

Palabras-clave
control biológico, hongo entomopatógeno, gusano blanco, escarabeido, patología de insectos

 
 en Evaluation of two Strains of Metarhizium Anisopliae var. Anisopliae (Metsh.) for Control of White Grub Hylamorpha Elegans Burm. (Coleoptera: Scarabaeidae) Larvae
Rodríguez, Marta; France, Andrés & Gerding, Macarena

Abstract

The white grub, Hylamorpha elegans Burm., is a serious pest on cereals and pastures in Chile. In laboratory and greenhouse studies, the pathogenicity of two Metarhizium anisopliae var. anisopliae (Metsh.) isolates: Qu-M270 and Qu-M802 on H. elegans larvae was evaluated. The first evaluation was carried out by immersion of third instar larvae in increasing suspensions, 0 to 108 conidia mL-1 of each isolate. The area under an accumulated mortality curve was calculated, observing differences (P = 0.032) between the isolates, with Qu-M270 being 50% superior to Qu-M802. The LC50 and LC90 for Qu-M270 were 104.7 and 107.5 conidia mL-1, respectively. The greenhouse evaluation was carried out in pots with pasteurized soil and 10 larvae of H. elegans, on which four suspensions (0 to 1014 conidia ha-1) were pulverized. After thirty days, there were no significant differences in the mortality between doses of 1013 and 1014 conidia ha-1 (P < 0.05), with 95 and 94% of larval mortality, respectively, but there was a difference at 1012 conidia ha-1 (P = 0.045) with 74% mortality. It is concluded that Qu-M270 was more effective in the control of H. elegans larvae.

Keywords
biological control, entomopathogenic fungi, white grub, scarabs, insect pathology.

 
© Copyright 2004 - Instituto de Investigaciones Agropecuarias, INIA (Chile). Free, full-text also available from http://www.inia.cl/at/agritec.htm
Alternative site location: http://www.inia.cl/at/agritec.htm

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