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Agricultura Técnica
Instituto de Investigaciones Agropecuarias, INIA
ISSN: 0365-2807
EISSN: 0365-2807
Vol. 66, No. 2, 2006, pp. 133-140
Bioline Code: at06014
Full paper language: Spanish
Document type: Research Article
Document available free of charge

Agricultura Técnica, Vol. 66, No. 2, 2006, pp. 133-140

 en Oral toxicity of six insecticides on Vespula germanica check for this species in other resources (F.) larvae in the laboratory
Hewstone, Nicole O.; Valenzuela, Jorge B. & Muñoz Sch., Carlos

Abstract

Nests of yellowjacket wasp, Vespula germanica check for this species in other resources F., were collected, from which comb pieces were selected with last stage larvae (n ≥ 35), that were later maintained at 25°C and ~50% RH in absence of light. Laboratory assays were conducted to determine the LC50 of commercial formulations of abamectin (applied in a range from 6.8 to 0.84 mg L-1), Bacillus thuringiensis check for this species in other resources (640-5.1 mg L-1), fipronil (1,000-1 mg L-1), methoxyfenozide (9,600-60 mg L-1), spinosad (106-0.11 mg L-1), and triflumuron (24,000-2.4 mg L-1). Each larva was fed 1,5 μL of insecticide solution (30% honey solution mixed with the commercial formulations, and a control was fed with only 30% honey solution). The symptoms of the neurotoxic insecticides previous to death were body paralysis and cuticle darkening within the first 72 h; triflumuron was effective, but because of its form of action its symptoms took longer to appear (incomplete ecdysis, and/or partial rupture of the cuticle). The LC50 were determined by Probit analyses, were: spinosad, 0.29 mg L-1; abamectin, 1.40 mg L-1; fipronil, 3.34 mg L-1, and triflumuron, 11.83 mg L-1. Bacillus thuringiensis had no lethal effect on larvae in the range of concentrations evaluated. With methoxyfenozide, mortality was obtained only when using the highest concentration (9,600 mg L-1).

Keywords
abamectin, Bacillus thuringiensis, German yellow jacket wasp, fipronil, methoxyfenozide, spinosad, triflumuron.

 
 es Toxicidad oral de seis insecticidas en larvas de Vespula germanica check for this species in other resources (F.) en laboratorio
Hewstone, Nicole O.; Valenzuela, Jorge B. & Muñoz Sch., Carlos

Resumen

Se colectaron nidos de la avispa chaqueta amarilla, Vespula germanica check for this species in other resources F., de los cuales se seleccionaron pisos con larvas de último estado (n ≥ 35), que se mantuvieron posteriormente a 25°C y ~50% HR en oscuridad. Se hicieron ensayos en laboratorio para determinar la CL50 de formulaciones comerciales de abamectina (aplicada en un rango de 6,8 a 0,84 mg L-1), Bacillus thuringiensis check for this species in other resources (640-5,1 mg L-1), fipronil (1.000-1 mg L-1), metoxifenocide (9.600-60 mg L-1), spinosad (106-0,11 mg L-1) y triflumuron (24.000-2,4 mg L-1). Cada larva se alimentó con 1,5 μL de solución insecticida (solución de 30% de miel mezclada con las formulaciones comerciales), más un piso control alimentado sólo con la solución de 30% de miel. Los síntomas de los insecticidas neurotóxicos previos a la muerte fueron parálisis corporal y pardeamiento de la cutícula dentro de las 72 h; el triflumuron fue efectivo, pero por su forma de acción demoró más en causar síntomas (ecdisis incompleta y/o ruptura parcial de la cutícula). Las CL50, estimadas por análisis Probit, fueron: spinosad, 0,29 mg L-1; abamectina, 1,40 mg L-1; fipronil, 3,34 mg L-1 y triflumuron, 11,83 mg L-1. Bacillus thuringiensis no tuvo efecto letal sobre las larvas en el rango de concentraciones evaluado. Con metoxifenocide se obtuvo mortalidad sólo al utilizar la concentración mayor (9.600 mg L-1).

Palabras-clave
abamectina, avispa chaqueta amarilla, Bacillus thuringiensis, fipronil, metoxifenocide, spinosad, triflumuron.

 
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