search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Australasian Biotechnology (backfiles)
AusBiotech
ISSN: 1036-7128
Vol. 6, No. 5, 1996
Bioline Code: au96015
Full paper language: English
Document type: Research Article
Document available free of charge

Australasian Biotechnology (backfiles), Vol. 6, No. 5, 1996

 en Nucleic acid sequence detection systems: revolutionary automation for monitoring and reporting PCR products

Abstract

Australasian Biotechnology, 
Volume 6 Number 5, September/October 1996, pp.285-294

Nucleic acid sequence detection systems: revolutionary
automation for monitoring and reporting PCR products

Brant J Bassam, Traci Allen, Susan Flood, Junko
Stevens, Paul Wyatt, and Kenneth J Livak

Code Number: AU96015
Size of Files:
    Text: 37.0K
    Graphics: Photographs (jpg) - 431.6K

Abstract

Recent technological advances at PE-Applied Biosystems have
made fully-automated real-time detection of specific PCR
products possible. Both the 7700 and 7200 sequence detection
systems integrate four major elements: (1) fluorogenic
chemistry for target-specific oligonucleotide probes; (2) an
exploitation of the polymerisation-dependant 5' nuclease
activity of the DNA polymerase; (3) instrumentation to measure
fluorescence signal within a closed PCR reaction vessel; (4)
software that processes and analyses the data. Dedicated
systems are available for simple end-point analysis of
accumulated PCR product and real-time analysis to accurately
determine initial target copy number. Real-time analysis also
allows the reaction kinetics of a PCR to be interrogated and
provides useful optimisation data. Multiple fluorophores are
used for signal normalisation, PCR controls, and multiplex
applications. These systems have been designed for
cost-effectiveness and high-throughput, and should
revolutionise nucleic acid sequence detection and genetic
screening studies.

Copyright 1996 Australian Biotechnology Association Ltd.

 

Alternative site location: http://www.ausbiotech.org/content.asp?pageid=16

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2020, Site last up-dated on 09-Nov-2020.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil