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Biotecnologia Aplicada
Elfos Scientiae
ISSN: 0684-4551
Vol. 17, No. 1, 2000, pp. 46-47
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Bioline Code: ba00011
Full paper language: English
Document type: Research Article
Document available free of charge
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Biotecnologia Aplicada, Vol. 17, No. 1, 2000, pp. 46-47
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New Approaches to Quantitative Proteome Analysis
Aebersold, Ruedi; Rist, Beate & Gygi, Steven P.
Abstract
With the completion of a rapidly increasing number of complete genomic sequences much attention is currently focused on the questions if and how the information contained in sequence databases can be interpreted in terms of the structure, function and control of biological systems. Quantitative proteome analysis, the global analysis of protein expression, has been proposed as a method to study genes at a steady state and after perturbation-induced changes. Here we discuss the justification for gene expression analysis at the protein level, highlight the limitations in the current standard proteome technology, and introduce a new experimental approach to quantitative proteome analysis.
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