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Biotecnologia Aplicada
Elfos Scientiae
ISSN: 0684-4551
Vol. 12, No. 3, 1995, pp. 166-167
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Bioline Code: ba95059
Full paper language: English
Document type: Report
Document available free of charge
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Biotecnologia Aplicada, Vol. 12, No. 3, 1995, pp. 166-167
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Expression and structural analysis of 14-3-3 proteins
Madrazo, Joel; Jones, David; Martin, Harry; Robinson, Karen; Nielsen, Peter; Roseboom, Patrick; Patel, Yasmina; Howell, Steven & Aitken, Alastair
Abstract
In this study we have used isoform-specific antibodies to analyse the domain structure of members of the 14-3-3 family after digestion with proteases. We concentrated on two isoforms of 14-3-3: tau, which is found at low levels in all tissues tested to date, and epsilon, which is found at high levels in brain and other tissues. Intact tau isoform and various deleted forms of tau were expressed in E. coli. Regions of the protein involved in dimerisation and membrane attachment were determined, and the nature of the phosphorilation by protein kinase C was analysed. In this way we have started to dissect the structure of 14-3-3 proteins and their function as regulators of protein kinase C.
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© Copyright 1995 Sociedad Iberolatinamericana de Biotecnologia Aplicada a la Salud Alternative site location: http://elfosscientiae.cigb.edu.cu/Archivo.asp?Id=6
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