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Biotecnologia Aplicada
Elfos Scientiae
ISSN: 0684-4551
Vol. 13, No. 2, 1996, pp. 98-100
Bioline Code: ba96039
Full paper language: English
Document type: Research Article
Document available free of charge

Biotecnologia Aplicada, Vol. 13, No. 2, 1996, pp. 98-100

 es
Vivero, Juan Manuel; Castorena-Cortes, Gladys; Meneses-Acosta, Angelica; Montero-Molina, Laura & Sanchez-Garcia, Lourdes

Resumen

Con el proposito de obtener las mejores condiciones para la acumulacion de ergometrina en suspensiones celulares de Ipomoea alba (Convolvulaceae) check for this species in other resources L., se utilizaron tres combinaciones diferentes de reguladores del crecimiento para determinar su efecto sobre la productividad y desarrollo de estas celulas. Las combinaciones evaluadas fueron: 0,453 uM 2,4-D 0,093 uM cinetina; 0,905 uM 2,4-D 0,093 uM cinetina y 1,357 uM 2,4-D 0,093 uM cinetina, utilizando con todas el compuesto basal de sales de Murashige y Skoog y 100 mg/mL de myo-inositol, 10 mg/mL de tiamina-HCl y 30 g/l sacarosa. El pH del medio se ajusto a 5,4 antes de la esterilizacion (121 C, 15 psi, 15 min). La primera de las tres combinaciones resulto ser la de productividad mas estable y consistente durante el experimento; por lo que se recomienda esta como un modelo inicial para la determinacion de los parametros productivos para el proceso de escalado.

Palabras-clave
ergometrina, cinetica de crecimiento, Ipomoea alba L.

 
 en Effect of 2.4-d concentration on ergometrine accumulation in Ipomoea alba (Convolvulaceae) check for this species in other resources L. cell suspension culture
Vivero, Juan Manuel; Castorena-Cortes, Gladys; Meneses-Acosta, Angelica; Montero-Molina, Laura & Sanchez-Garcia, Lourdes

Abstract

In order to find the best conditions for ergometrine accumulation in cellular suspensions of Ipomoea alba (Convolvulaceae) check for this species in other resources L., three different combinations of growth regulators were tested to determine their effect on the productivity and kinetic performance of these cells. The combinations studied for this were 0.453 uM 2.4-D 0.093 uM kinetin, 0.905 uM 2.4-D 0.093 uM kinetin and 1.357 uM 2.4-D 0.093 uM kinetin, all of them with the Murashige and Skoog basal salt mixture (1) with 100mg/mL myo-inositol, 10 mg/mL thiamine-HCl and 30 g/l sucrose added. The pH of the medium was adjusted to 5.4 before sterilization (121 C, 15 psi, 15 min). The first of the three combinations showed to be the one with the more stable and consistent productivity during the experiment; thus, it is recommended as an initial model for the determination of production parameters for scaling-up.

Keywords
ergometrine, growing kinetic, Ipomoea alba L.

 
© Copyright 1996 Elfos Scientiae
Alternative site location: http://elfosscientiae.cigb.edu.cu/Archivo.asp?Id=6

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