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Biotecnologia Aplicada
Elfos Scientiae
ISSN: 0684-4551
Vol. 14, No. 2, 1997, pp. 106-110
Bioline Code: ba97025
Full paper language: English
Document type: Research Article
Document available free of charge

Biotecnologia Aplicada, Vol. 14, No. 2, 1997, pp. 106-110

 en Production of monoclonal antibodies cb ifn 2.4 in the hollow fiber bioreactors ACUSYST-R and SACCEL
Perez, Mayte; Valdes, Israel; Pierrat, Ricardo; Garcia, Cristina; Gonzalez, Marcos; de la Torre, Daisy; Aleman, Maria del Rosario & Valdes, Rodolfo

Abstract

Monoclonal antibodies (MAbs) are produced in mice by the intraperitoneal injection of the MAb-producing hybridoma cells. The laws for animal protection and the regulations required for the production of MAbs for human use are more and more strict and have lead to develop new techniques to obtain these molecules. In this paper we compare a new bioreactor system: the cell culture automatic system (SACCEL) (ICID, Havana) with a well known commercial system: ACUSYST-R (Endotronics, Minneappolis, USA). Taking into account quality, subclass and immunopurification performance we did not obtain significant differences regarding the bulk harvest yield and MAb quality when we used the MAb CB IFN 2.4. We reduced the medium pump rate in relation to MAb production and found that even a 50 % reduction of the culture medium consumption did not affect the specific antibody production. This result represents a 13 % reduction of the production cost per unit.

Keywords
SACCEL, ACUSYST-R, hybridoma, monoclonal antibody, hollow fiber

 
 es
Perez, Mayte; Valdes, Israel; Pierrat, Ricardo; Garcia, Cristina; Gonzalez, Marcos; de la Torre, Daisy; Aleman, Maria del Rosario & Valdes, Rodolfo

Resumen

Los anticuerpos monoclonales (AcMs) se obtienen en ratones mediante la inyeccion intraperitonial de hibridomas productores de AcMs. Las leyes para la proteccion de animales, y los elementos regulatorios requeridos para la produccion de estos para uso humano son cada vez mas estrictos, por lo que se han desarrollado nuevas tecnicas de produccion: el cultivo celular en sistema de fibra hueca. En este trabajo comparamos un nuevo biorreactor: SACCEL, (ICID, Habana) con el sistema comercial ACUSYST-R (Endotronics, Minneappolis, USA). La cosecha, subclase, comportamiento en inmunopurificacion y calidad del AcM fueron similares cuando se utilizo el AcM CB IFN 2,4. Se redujo el consumo de medio de cultivo estableciendo una estrategia de suministro programado de glucosa. Se comprobo que un 50 % de reduccion de este medio no afecta la produccion de anticuerpos para la linea celular CB IFN 2.4. Este resultado representa una reduccion de los costos de produccion por unidad de un 13 % respecto a corridas anteriores utilizando biorreactores ACUSYST-R.

Palabras-clave
SACCEL, ACCUSYST-R, hibridoma, anticuerpo monoclonal, fibra hueca

 
© Copyright 1997 Elfos Scientiae
Alternative site location: http://elfosscientiae.cigb.edu.cu/Archivo.asp?Id=6

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