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Biotecnologia Aplicada
Elfos Scientiae
ISSN: 0684-4551
Vol. 14, No. 3, 1997, pp. 149-161
Bioline Code: ba97037
Full paper language: Spanish
Document type: Research Article
Document available free of charge

Biotecnologia Aplicada, Vol. 14, No. 3, 1997, pp. 149-161

 es Transfeccion de adn a celulas de mamiferos
Castro, Fidel Ovidio & Portelles, Yangtse

Resumen

La transfeccion o introduccion de material genetico exogeno a celulas en cultivo es una eficaz herramienta en los estudios de biologia molecular y celular. Mediante las diferentes tecnicas de transfeccion se puede alterar establemente el genoma de una celula para que produzca una proteina recombinante o evaluar de forma rapida y transitoria la fortaleza de un promotor acoplado a un gen reportero, entre otras muchas aplicaciones. Las distintas tecnicas de transfeccion empleadas para celulas de mamiferos son discutidas en esta revision, sobre la base de sus atributos y desventajas. Tambien son abordados los sistemas de genes reporteros mas utilizados en transfecciones transientes. Se concluye que cada metodo de transfeccion debe ser optimizado para una linea celular dada.

Palabras-clave
transfeccion, GFP, polietilenimina, celulas de mamiferos, transgenesis

 
 en
Castro, Fidel Ovidio & Portelles, Yangtse

Abstract

Transfection of foreign DNA into living cells in vitro is a powerful research tool in both molecular and cell biology. By means of the different transfection techniques available at present, one can stably change the genetic make-up of a cell as to produce recombinant proteins from it, or test the strenght of a gene promoter coupled to a reporter gene, among many other applications. In this review are discussed the most important transfection techniques with special emphasis in advantages and disadvantages of each one. Reporter gene systems for use in eukaryotic cells are also presented and discussed. As an important conclusion, it is noted that any given transfection protocol needs to be optimized for a given cell line.

Keywords
transfection, GFP, polyethilenimine, mammalian cells, transgenesis

 
© Copyright 1997 Elfos Scientiae
Alternative site location: http://elfosscientiae.cigb.edu.cu/Archivo.asp?Id=6

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