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Biotecnologia Aplicada
Elfos Scientiae
ISSN: 0684-4551
Vol. 14, No. 4, 1997, pp. 237-241
Bioline Code: ba97053
Full paper language: English
Document type: Research Article
Document available free of charge

Biotecnologia Aplicada, Vol. 14, No. 4, 1997, pp. 237-241

 es
Baker, Roberto Basulto; Aguilera, Lesvia Calzada; Gutierrez, Tania Olivera & de la Fuente Garcia, Jose

Resumen

Se describe el uso de sondas de ADN especificas en la identificacion de los genes que codifican los antigenos fimbriales K88, K99, F41 y 987P en 142 aislamientos de Escherichia coli check for this species in other resources de animales con diarrea (117 de cerdos y 25 de terneros). Diez aislamientos de cerdos resultaron positivos con las sondas utilizadas: cinco K88+, cuatro 987P+ y una K99+F41+, resultando todos ellos fenotipicamente positivos en el ensayo para la expresion de antigenos fimbriales por el sistema inmunoenzimatico. En este estudio, el 13 % de los cerdos neonatos analizados resulto ser portador de cepas de E. coli K88+, K99+F41+ y 987P+. Con el empleo de sondas de ADN se reporta por primera vez en Cuba la existencia de cepas autoctonas 987P+ y K99+F41+ en cerdos neonatos con diarrea. Ninguno de los aislamientos de E. coli en terneros resulto positivo para K99 y/o F41.

Palabras-clave
Escherichia coli enterotoxigenica, antigeno fimbrial, diarrea

 
 en Detection of genes for fimbrial antigens in Escherichia coli check for this species in other resources isolated from piglets with diarrhea in Camaguey
Baker, Roberto Basulto; Aguilera, Lesvia Calzada; Gutierrez, Tania Olivera & de la Fuente Garcia, Jose

Abstract

Specific DNA probes for genes encoding K88, K99, F41 and 987P fimbrial antigens were used to examine 142 Escherichia coli check for this species in other resources isolates from diarrheal cases (117 from pigs and 25 from calves). Ten isolates from pigs were adhesing gene positive with the DNA probes used: five K88+, four 987P+ and one K99+F41+. All were phenotypically positive when assayed for the expression of fimbrial antigens by enzyme-linked immunoabsorbent assays. In this study, 13 % of the pigs were infected by K88+, K99+F41+ and 987P+ E. coli strains. The existence for the first time in Cuba of 987P+ and K99+F41+ native strains in pigs with diarrhea, detected with DNA probes, is reported. None of the isolates from calves resulted positive for K99 and/or F41.

Keywords
enterotoxigenic Escherichia coli, fimbrial antigen, diarrhea

 
© Copyright 1997 Elfos Scientiae
Alternative site location: http://elfosscientiae.cigb.edu.cu/Archivo.asp?Id=6

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