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Biotecnologia Aplicada
Elfos Scientiae
ISSN: 0684-4551
Vol. 16, No. 3, 1999
Bioline Code: ba99026
Full paper language: English
Document type: Research Article
Document available free of charge

Biotecnologia Aplicada, Vol. 16, No. 3, 1999

 en Molecular Characterization of Recombinant Human Interferon Alpha-2b Produced in Cuba

Abstract

Biotecnologia Aplicada 1999; Vol. 16 No. 3, pp. 154-159

Molecular Characterization of Recombinant Human Interferon Alpha-2b Produced in Cuba

Héctor Santana, Eduardo Martínez, Julio C Sánchez, Galina Moya, Raudel Sosa, Eugenio Hardy,
Alejandro Beldarraín, Vivian Huerta, Luis J González, Lázaro Betancourt, Vladimir Besada,
Rudka Brito, Tania Currás, Joel Ferrero, Vivian Pujols, Miriela Gil, Luis Herrera,

Code Number: BA99026

ABSTRACT

The recombinant human interferon a 2b (IFN-a 2b) produced by Cuban technology is obtained from Escherichia coli. This is the active principle of the product registered in Cuba as Heberon alfa R® trademark (IFN-a 2b, CIGB, Havana), which has been successfully used worldwide for the therapy of several viral diseases and neoplasms. Here we describe the purity and identity tests used for its molecular characterization. The data show a product with a well-established identity, a high purity and a specific activity higher than 1.4 x 108 IU/mg of proteins. We also compared the final preparation with other IFN-a 2 products available in the international market. It behaved very similar to Intron A® and Roferon A®, and showed a higher homogeneity when compared with Bioferon® and Interimmun®.

Keywords: IFN, molecular characterization, recombinant human interferon alfa 2b

RESUMEN

El interferón a 2b humano recombinante (IFN-a 2b) producido mediante la tecnología cubana es obtenido a partir de Escherichia coli. Éste constituye el principio activo del producto registrado en Cuba con la marca comercial Heberon alfa R® (IFN-a 2b, CIGB, La Habana), el cual ha sido usado exitosamente en muchos lugares del mundo para la terapia de varias enfermedades virales y neoplasias. En este trabajo se describen los ensayos de pureza e identidad para su caracterización molecular. Los datos muestran un producto de identidad bien establecida, de alta pureza y de actividad específica mayor que 1,4 x 108 UI/mg de proteínas. Además, se comparó la preparación final con otros productos IFN-a 2 similares distribuidos en el mercado internacional. El interferón cubano mostró características muy similares a Intron A® y Roferon A®, y mayor homogeneidad cuando se comparó con Bioferon® e Interimmun®.

Palabras claves: caracterización molecular, IFN, interferón alfa 2b humano recombinante

Copyright 1999 Elfos Scientiae

 

Alternative site location: http://elfosscientiae.cigb.edu.cu/Archivo.asp?Id=6

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