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Biotecnologia Aplicada
Elfos Scientiae
ISSN: 0684-4551
Vol. 16, No. 3, 1999
Bioline Code: ba99028
Full paper language: English
Document type: Research Article
Document available free of charge

Biotecnologia Aplicada, Vol. 16, No. 3, 1999

 en Cloning and Sequencing of the 5' Region from Human Spinocerebellar Ataxia 2 Gene

Abstract

Biotecnologia Aplicada 1999; Vol. 16 No. 3, pp. 165-168

Cloning and Sequencing of the 5' Region from Human Spinocerebellar Ataxia 2 Gene

Jorge Aguiar, María Vázquez, Julio Fernández, Yssel Mendoza, George Auburger

Code Number: BA99028

ABSTRACT

A restriction fragment containing the 5' region of the spinocerebellar ataxia type 2 (SCA2) gene was subcloned from PAC65i22 into pBluescript and totally sequenced using an ABI 373A automated sequencer, in combination with the direct sequencing of the mentioned PAC genomic clone through the DOP-vector PCR method. A typical CpG island with several potential sites for Sp1 transcription factor has been found, where the promoter activity of the SCA2 gene can be situated, maybe like it occurs with the gene for Huntington disease.

Keywords: CpG island, disease, spinocerebellar ataxia 2, transgenic mouse

RESUMEN

Un fragmento de restricción que contiene la región 5' del gen de la ataxia espinocerebelosa 2 (SCA2), fue subclonado en el vector pBluescript a partir del PAC65i22, y secuenciado completamente mediante el empleo de un secuenciador automatizado ABI 373A, en combinación con la secuenciación directa del mencionado clon genómico de PAC mediante la técnica de PCR basada en el vector DOP. Se encontró un islote de CpG típico con varios sitios potenciales de unión al factor de transcripción Sp1, donde puede estar situada la actividad promotora del gen de la SCA2, como ocurre posiblemente para el gen de la enfermedad de Huntington.

Palabras claves: ataxia espinocerebelosa 2, enfermedad, islote de CpG, ratón transgénico

Copyright 1999 Elfos Scientiae

 

Alternative site location: http://elfosscientiae.cigb.edu.cu/Archivo.asp?Id=6

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