Biotecnologia Aplicada 1999; Vol. 16 No. 3, pp. 173-175
Use of Poly-L-lysine Precoating in an ELISA for
the Detection of Antibodies against Serogroup C Neisseria
meningitidis Capsular Polysaccharide
Rolando Ochoa Azze, Mayte Nerey Olivares, Juan C Martínez
Rodríguez
Code Number: BA99030
Abstract
Precoating with poly-L-lysine was analyzed in an ELISA used
for the quantitative determination of antibodies against serogroup
C Neisseria meningitidis capsular polysaccharide. Different
coatings were analyzed: purified capsular polysaccharide of
serogroup C N. meningitidis did not attach well to
polystyrene, but precoating with poly-L-lysine and the use of
methylated human serum albumin attached to polysaccharide had a
good performance. Cross reactivity to poly-L-lysine was not
detected in 48 adults immunized with a divalent (serogroups B
and C) meningococcal vaccine (VA-MENGOC-BC®).
Seroconversion and seroresponse were detected in 89.58 and 97.92%
of the vaccinated individuals, respectively.
Keywords: ELISA for anti-polysaccharide C antibodies,
poly-L-lysine, poly-L-lysine precoating, serogroup C Neisseria
meningitidis
Resumen
Se evaluó el prerecubrimiento con poli-L-lisina en un
ELISA para la cuantificación de anticuerpos contra el
polisacárido capsular de Neisseria meningitidis
serogrupo C. Se analizaron diferentes recubrimientos: el
polisacárido C no se unió bien al poliestireno, pero
se alcanzaron buenos resultados recubriendo previamente con
poli-L-lisina, así como con seroalbúmina humana
metilada unida al polisacárido C. No se detectó
reactividad cruzada a la poli-L-lisina en 48 adultos inmunizados
con una vacuna anti-meningocóccica bivalente contra los
serogrupos B y C (VA-MENGOC-BC®). Se detectó
seroconversión en 89,58% de los vacunados y
serorrespondió 97,92%.
Palabras claves: ELISA para anticuerpos
anti-polisacárido C, Neisseria meningitidis serogrupo
C, poli-L-lisina, prerrecubrimiento con poli-L-lisina
Copyright 1999 Elfos Scientiae
