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Biotecnologia Aplicada
Elfos Scientiae
ISSN: 0684-4551
Vol. 16, No. 3, 1999
Bioline Code: ba99030
Full paper language: English
Document type: Research Article
Document available free of charge

Biotecnologia Aplicada, Vol. 16, No. 3, 1999

 en Use of Poly-L-lysine Precoating in an ELISA for the Detection of Antibodies against Serogroup C Neisseria meningitidis Capsular Polysaccharide

Abstract

Biotecnologia Aplicada 1999; Vol. 16 No. 3, pp. 173-175

Use of Poly-L-lysine Precoating in an ELISA for the Detection of Antibodies against Serogroup C Neisseria meningitidis Capsular Polysaccharide

Rolando Ochoa Azze, Mayte Nerey Olivares, Juan C Martínez Rodríguez

Code Number: BA99030

Abstract

Precoating with poly-L-lysine was analyzed in an ELISA used for the quantitative determination of antibodies against serogroup C Neisseria meningitidis capsular polysaccharide. Different coatings were analyzed: purified capsular polysaccharide of serogroup C N. meningitidis did not attach well to polystyrene, but precoating with poly-L-lysine and the use of methylated human serum albumin attached to polysaccharide had a good performance. Cross reactivity to poly-L-lysine was not detected in 48 adults immunized with a divalent (serogroups B and C) meningococcal vaccine (VA-MENGOC-BC®). Seroconversion and seroresponse were detected in 89.58 and 97.92% of the vaccinated individuals, respectively.

Keywords: ELISA for anti-polysaccharide C antibodies, poly-L-lysine, poly-L-lysine precoating, serogroup C Neisseria meningitidis

Resumen

Se evaluó el prerecubrimiento con poli-L-lisina en un ELISA para la cuantificación de anticuerpos contra el polisacárido capsular de Neisseria meningitidis serogrupo C. Se analizaron diferentes recubrimientos: el polisacárido C no se unió bien al poliestireno, pero se alcanzaron buenos resultados recubriendo previamente con poli-L-lisina, así como con seroalbúmina humana metilada unida al polisacárido C. No se detectó reactividad cruzada a la poli-L-lisina en 48 adultos inmunizados con una vacuna anti-meningocóccica bivalente contra los serogrupos B y C (VA-MENGOC-BC®). Se detectó seroconversión en 89,58% de los vacunados y serorrespondió 97,92%.

Palabras claves: ELISA para anticuerpos anti-polisacárido C, Neisseria meningitidis serogrupo C, poli-L-lisina, prerrecubrimiento con poli-L-lisina

Copyright 1999 Elfos Scientiae

 

Alternative site location: http://elfosscientiae.cigb.edu.cu/Archivo.asp?Id=6

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