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Brazilian Journal in Health Promotion
Fortaleza University
ISSN: 1806-1222
EISSN: 1806-1222
Vol. 25, No. 2, 2012, pp. 197-201
Bioline Code: bh12029
Full paper language: Portuguese
Document type: Research Article
Document available free of charge

Brazilian Journal in Health Promotion, Vol. 25, No. 2, 2012, pp. 197-201

 pt EFEITO ANTIMICROBIANO DE TINTURAS DE PRODUTOS NATURAIS SOBRE BACTÉRIAS DA CÁRIE DENTÁRIA
Pinheiro, Mayara Abreu; Brito, Deborah Brindeiro de Araújo; de Almeida, Leopoldina Fátima Dantas; Cavalcanti, Yuri Wanderley & Padilha, Wilton Wilney Nascimento

Resumo

Objetivo: Avaliar as atividades bacteriostatica e bactericida de tinturas de Rosmarinus officinalis check for this species in other resources (Alecrim), Calendula officinalis check for this species in other resources (Calendula) e Mikania glomerata check for this species in other resources (Guaco) sobre bacterias da carie dentaria e compara-las as da clorexidina.
Métodos: A Concentracao Inibitoria Minima (CIM) foi determinada pela tecnica da microdiluicao, utilizando microplacas de 96 furos. Em cada poco foram inseridos 10μL do inoculo (108 celulas/mL), 100μL de caldo BHI duplamente concentrado e 100μL das tinturas, em concentracoes que variaram de 100 a 0,78mg/mL, para T1, T2 e T3. Como controle positivo foi utilizado a Clorexidina 0,12%. A leitura foi feita apos 24 horas, pelo metodo visual. Os ensaios foram realizados em triplicata. A Concentracao Bactericida Minima (CBM) foi obtida pela semeadura da ultima concentracao bacteriostatica e da sua anterior, para cada produto, em meio Agar Sangue. Foram consideradas CBM as placas que nao apresentaram crescimento bacteriano.
Resultados: Sobre Streptococcus mutans check for this species in other resources , verificou-se CIM de 6,25mg/mL, para T1, T2 e T3 e CBM de 12,5mg/mL para T1 e T2 e de 6,25mg/mL para T3. Sobre Streptococcus oralis check for this species in other resources todos os produtos apresentaram CIM e CBM de 0,78mg/mL. Para clorexidina, a CIM e CBM foram 0,04mg/mL, sobre S. mutans e S. oralis.
Conclusão: As tinturas de Alecrim, Calendula e Guaco apresentaram acao bactericida e bacteriostatica em baixas concentracoes, sobre Streptococcus mutans e Streptococcus oralis, entretanto a atividade antimicrobiana da Clorexidina foi superior a das tinturas.

Palavras-chave
Placa Dentária; Streptococcus mutans; Streptococcus oralis; Produtos Biológicos

 
 en Antimicrobial effect of natural dyeing products upon decay-causing bacteria
Pinheiro, Mayara Abreu; Brito, Deborah Brindeiro de Araújo; de Almeida, Leopoldina Fátima Dantas; Cavalcanti, Yuri Wanderley & Padilha, Wilton Wilney Nascimento

Abstract

Objective: To evaluate the bacteriostatic and bactericidal activities of tinctures of Rosmarinus officinalis check for this species in other resources (Rosemary), Calendula officinalis check for this species in other resources (Marigold) and Mikania glomerata check for this species in other resources (Guaco) upon decay-causing bacteria and compare them to chlorhexidine’s.
Methods: The Minimum Inhibitory Concentration (MIC) was determined by microdilution technique, using 96-well microplates. In each well were inserted 10μL of inoculum (108 cells/mL), 100μL of doubly concentrated BHI broth and 100μL of tinctures at concentrations that vary between 100 and 0.78 mg/mL for T1, T2 and T3. As positive control, Chlorhexidine 0.12% was used. Reading was performed after 24 hours, by visual method. The assays were realized in triplicate. Minimum Bactericidal Concentration (MBC) was obtained by seeding of last bacteriostatic and its preceding concentrations, for each product, on Blood Agar. The MBC was considered in the plates that did not present any bacterial growth.
Results: Upon Streptococcus mutans check for this species in other resources , MIC was verified at 6.25mg/mL, for T1, T2, and T3 and MBC at 12.5mg/mL for T1 and T2, and at 6.25mg/mL for T3. Upon Streptococcus oralis check for this species in other resources , all products presented MIC and MBC at 0.78mg/mL. For Chlorhexidine, the MIC and MBC were 0,04mg/mL, upon S. mutans and S. oralis.
Conclusion: The tinctures of Rosemary, Marigold and Guaco showed bactericidal and bacteriostatic activities at low concentrations, upon Streptococcus mutans e Streptococcus oralis. However, the antimicrobial activity of chlorhexidine was superior to the tinctures’.

Keywords
Dental Plaque; Streptococcus mutans; Streptococcus oralis; Biological Agents

 
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