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Biokemistri
Nigerian Society for Experimental Biology
ISSN: 0795-8080
Vol. 18, No. 1, 2006, pp. 15-20
Bioline Code: bk06003
Full paper language: English
Document type: Research Article
Document available free of charge

Biokemistri, Vol. 18, No. 1, 2006, pp. 15-20

 en Comparison Of α-amylase Activity In Some Sprouting Nigerian Cereals
Egwim, Evans C. & Oloyede, Oyelola B.

Abstract

The yield and characteristics of Alpha-amylase obtained from some sprouting Nigeria cereals (maize, acha, rice and sorghum) were compared using standard procedures. The result indicated maximum α-amylase yield was attained from 72-120 hours of sprouting cereals). Of all the sprouted cereals, acha gave rise to the highest amylase yield (16.6 ± 0.02x102 and the least was exhibited by maize (11.0±0.04x102). The optimum temperature for α-amylase activity was 60°C for maize and rice while that of acha and sorghum was 70°C. The optimal pH for the activity of α-amylase from sprouting maize, rice, acha, and sorghum were exhibited at 6.5, 5.5, 6.5 and 5.8 respectively. The apparent kinetic parameters, Vmax and Km, were 9.0x10-2 and 0.23; 12.5x10-2 and 0.56; 7.0x10-2 and 0.29; 12.5x10-2 and 0.50 for α-amylase from sprouting maize, acha, rice and sorghum respectively. It is concluded that α-amylase obtained from sprouting Nigeria cereals manifest differences in quality and quantity, and may be useful in different starch processing industries.

Keywords
cereals, α-amylase, sprouting, apparent kinetics, acha

 
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