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Biokemistri
Nigerian Society for Experimental Biology
ISSN: 0795-8080
Vol. 23, No. 1, 2011, pp. 1-8
Bioline Code: bk11001
Full paper language: English
Document type: Research Article
Document available free of charge

Biokemistri, Vol. 23, No. 1, 2011, pp. 1-8

 en Laboratory scale production of glucose syrup by the enzymatic hydrolysis of starch made from maize, millet and sorghum
Zainab, A.; Modu, S.; Falmata, A. S. & Maisaratu

Abstract

Glucose syrup production from maize, millet and sorghum starch was investigated. The starch extracted from yellow maize after steeping for 72hrs and purified by sedimentation method had the greatest yield of 86.71% (4.34 ± 0.37g) followed by the starch made from millet and sorghum with a yield of 65.94% (3.30 ± 0.25g) and 64.71% (3.23 ± 0.09g), respectively. The lowest gelatinization onset temperature of 59.25 ± 0.90°C was observed with sorghum starch. The glucose recovered with pure amyloglucosidase from Rhizopus check for this species in other resources mold produced 17.15 ± 0.10 mg/ml reducing sugar from yellow maize starch after 10 minutes. Millet and sorghum starch followed with a yield of 15.79 ± 0.20mg/ml and 11.32 ± 0.26mg/ml in 10 minutes, respectively. Liquid glucose produced with the pure amyloglucosidase revealed a dextrose equivalent of 78.28 ± 0.57%, 73.50 ± 0.66% and 65.66 ± 0.61% for sorghum, yellow maize and millet, respectively. Sorghum and yellow maize starch exhibited good potential as substrates for glucose syrup production.

Keywords
Glucose syrup; Enzymatic hydrolysis; Amyloglucosidase; Starch.

 
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