search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Biokemistri
Nigerian Society for Experimental Biology
ISSN: 0795-8080
Vol. 25, No. 2, 2013, pp. 72-78
Bioline Code: bk13012
Full paper language: English
Document type: Research Article
Document available free of charge

Biokemistri, Vol. 25, No. 2, 2013, pp. 72-78

 en Toxicity screening and in vitro antioxidant activities of aqueous extracts of Morinda lucida check for this species in other resources and Saccharum officinarum check for this species in other resources leaves
Ojewunmi, Oyesola; Oshodi, Tope; Ogundele, Omobolanle; Chijioke, Micah & Adenekan, Sunday

Abstract

The use of medicinal plants as natural antioxidants in the treatment of diabetes and cardiovascular diseases is on the increase. However, toxicity of the plants is also a major concern. Hence, this present study investigated the toxic and in vitro antioxidant activities of aqueous extracts of Morinda lucida check for this species in other resources and Saccharum officinarum check for this species in other resources leaves. Brine shrimp lethality assay for larvae nauplii was used to evaluate the toxicity of the two plant extracts. The antioxidant potential of the plant extracts was examined by determining tannin content, total flavonoid content, total antioxidant capacity, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, in vitro lipid peroxidation, and nitric oxide (NO) scavenging assay. Both Morinda lucida and Saccharum officinarum showed the presence of tannin and flavonoids; additional presence of saponin, alkaloid and phenol were confirmed in Morinda lucida while the presence of steroids was shown in Saccharum officinarum. Brine shrimp toxicity test revealed LC50 of 112.2 µg/ml, 44,668.3 µg/ml, and 14.12 µg/ml for Morinda lucida, Saccharum officinarum, and the standard (Potassium dichromate) respectively. The amount of total flavonoid content was 23.08 mg per 100 g and 5.28 mg per 100 g for M. lucida and S. officinarum respectively. Total antioxidant capacity assay showed 83.15 mg per 100 g for M. lucida and 27.56 mg per 100 g for S. officinarum. Morinda lucida and Saccharum officinarum showed IC50 values of 31.25 µg/ml and 68.75 µg/ml for DPPH free radical respectively. In Nitric oxide scavenging activities, IC50 values of Morinda lucida and Saccharum officinarum were 22.50 µg/ml and 21.88 µg/ml. It can be concluded that M. lucida and S. officinarum are not toxic and possess antioxidant activities.

Keywords
DPPH quenching; brine shrimp; antioxidants; phytochemical screening; Morinda lucida; Saccharum officinarum

 
© Copyright 2013 - Nigerian Society for Experimental Biology

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 16-Oct-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil