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Biokemistri
Nigerian Society for Experimental Biology
ISSN: 0795-8080
Vol. 25, No. 3, 2013, pp. 124-126
Bioline Code: bk13023
Full paper language: English
Document type: Short Communication
Document available free of charge

Biokemistri, Vol. 25, No. 3, 2013, pp. 124-126

 en Acute toxicity study on aqueous extract of the leaf of Cassia sieberiana check for this species in other resources D.C. ( Caesalpiniaceae check for this species in other resources ) in albino rats
Biu, Abdullahi Abubakar; Buratai, Lawan Bala; Konto, Mohammed; Luka, Joshua & Hauwa, Muhammed Mundu

Abstract

In order to evaluate the toxicity of Cassia sieberiana check for this species in other resources leaf extract in albino rats with the aim of establishing its safe application in experimental and field trials, the acute toxicity of the aqueous extract of the leaves was studied in albino rats. Four experimental groups of albino rats (A, B, C, D) were respectively given intraperitoneal doses of 400, 800, 1600 and 3200 mg/kg body weight of the extract, while the fifth group (E) was control. The LD50 was calculated, clinical signs and gross and microscopic lesions of the liver and kidney were recorded. Immediate clinical signs observed were weakness, depression, starry hair coat, anorexia, while abnormal gait, lordosis, opthalmia, coma and death appeared after 2 hours. Hepatomegaly and focal necrosis were observed on gross examination, while sinusoidal congestion with periportal necrosis and Kupffer cell proliferation were the microscopic lesions observed. Gross enlargement of the kidneys, vascular degeneration, and interstitial mononuclear cell infiltration were observed. The calculated median lethal dose (LD50) was 960 mg/kg, and the severity of clinical signs and hepatic and kidney lesions was dose dependent. This study has shown that Cassia sieberiana leaf aqueous extract could be toxic in a dose-dependent way, and should be used with caution in veterinary practice.

Keywords
Cassia sieberiana; Leaf aqueous extract; acute toxicity; albino rats

 
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