search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Biokemistri
Nigerian Society for Experimental Biology
ISSN: 0795-8080
Vol. 26, No. 1, 2014, pp. 19-24
Bioline Code: bk14004
Full paper language: English
Document type: Research Article
Document available free of charge

Biokemistri, Vol. 26, No. 1, 2014, pp. 19-24

 en Purification and characterization of thermostable glucoamylase from Rhizopus oligosporus check for this species in other resources SK5 mutant obtained through UV radiation and chemical mutagenesis
Kareem, Sarafadeen Olateju; Akpan, Inyang; Popoola, Temitope Olukayode Sunday & Sanni, Lateef Oladimeji

Abstract

Thermostable glucoamylase from Rhizopus oligosporus check for this species in other resources SK5 mutant was purified in a 3-step purification using Imarsil, activated charcoal and Sephadex-G-100 to achieve a 40-fold purification. The enzyme was optimally active at pH 5.0 and temperature of 80 °C. It exhibited a half-life of 60 minutes at 70 °C. Its stability was enhanced with addition of Soyabean flour or starch (3% w/w) leading to a retention of over 90% residual activity at 4 °C and 28 °C after 12 weeks of storage. SDS-PAGE analysis of purified enzyme showed two major bands with corresponding molecular weights of 36 kDa and 50 kDa. The study presents thermostable glucoamylase from Rhizopus oligosporus SK5 as a potential in the bioconversion of starch to glucose.

Keywords
Enzyme purification; thermostability; Rhizopus oligosporus; glucoamylase

 
© Copyright 2014 - Nigerian Society for Experimental Biology

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 05-Dec-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil