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Biokemistri
Nigerian Society for Experimental Biology
ISSN: 0795-8080
Vol. 27, No. 1, 2015, pp. 33-38
Bioline Code: bk15006
Full paper language: English
Document type: Research Article
Document available free of charge

Biokemistri, Vol. 27, No. 1, 2015, pp. 33-38

 en Partial characterization of lignin peroxidase expressed by bacterial and fungal isolates from termite gut
Egwim, Evans C.; Kabiru, Adamu Y. & Tola, Adeshola J.

Abstract

Lignin peroxidase producing microorganisms were isolated from the gut of Macrotermes nigeriense (Soldier and worker termite). The microorganisms isolated were Staphylococcus aureus check for this species in other resources , Staphylococcus epidermis check for this species in other resources , Bacillus subtilis check for this species in other resources , Micrococcus luteus check for this species in other resources , Epidermophyton flocussum check for this species in other resources , Microsporum distortum check for this species in other resources , Trichophyton megininii check for this species in other resources , Trichophyton mentagrophytes check for this species in other resources and Aspergillus niger check for this species in other resources . When the isolates were incubated for 24 hours in the presence of 0.5 w/v pyrogallol to test for the expression of peroxidase activities, B. subtilis produced the highest activity among the bacterial isolates, with optimum temperature of 35 °C, and optimum pH of 6.5. Similar screen among the fungal isolates found M. distortum to express the most activities, with optimum temperature of 40 °C, and optimum pH of 5.0, after 72 hours incubation. The results from this study suggest that B. subtilis and M. distortum can be a cheap source of lignin peroxidase for large scale commercial production, biotechnological and industrial applications.

Keywords
Macrotermes nigeriense; lignin peroxidase; microorganisms; enzyme activity

 
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