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Biokemistri
Nigerian Society for Experimental Biology
ISSN: 0795-8080
Vol. 27, No. 3, 2015, pp. 123-128
Bioline Code: bk15018
Full paper language: English
Document type: Research Article
Document available free of charge

Biokemistri, Vol. 27, No. 3, 2015, pp. 123-128

 en Interference of phenol during quantification of a bacterial lipoprotein
Lakshmikanth, Chikkamenahalli L.; Jacob, Shancy P.; Divya, Armugam; Rao, Appu Rao G. Appu & Gopal, Marathe K.

Abstract

Accurate protein estimation is an essential requirement for any biochemical investigation. The bacterial Braun liporotein (BLP) from E. coli check for this species in other resources (a Toll-2 receptor ligand) is purified via phenol extraction on the basis of selective extraction of the lipoprotein. The procedure leaves behind the major endotoxin lipopolysaccharide (LPS) that acts through the related Toll-4 receptor. However, as low as 0.00001% of phenol carried over during lipoprotein isolation interferes in the Lowry’s method of protein estimation. A simple gel filtration on sephadex G-50 efficiently separates lipoproteins from phenol thereby avoiding inaccurate protein estimation of the lipoprotein content and making it suitable ligand for Toll-2 receptor.

Keywords
Lipoproteins; Lipopolysaccharide (LPS); Lowry's method; Phenol interference

 
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