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Journal of Culture Collections
National Bank for Industrial Microorganisms and Cell Cultures
ISSN: 1310-8360
Vol. 3, No. 1, 2002, pp. 53-58
Bioline Code: cc02008
Full paper language: English
Document type: Research Article
Document available free of charge

Journal of Culture Collections, Vol. 3, No. 1, 2002, pp. 53-58

 en ENCAPSULATION OF LACTIC ACID BACTERIA IN CALCIUM ALGINATE BEADS FOR BACTERIOCIN PRODUCTON
Evelina Ivanova, Valentina Chipeva, Iskra Ivanova, Xavier Dousset and Denis Poncelet

Abstract

Investigations on encapsulation of lactic acid bacteria in alginate beads were carried out. For this purpose a bacteriocin producing strain Enterococcus faecium check for this species in other resources A 2000 was used. For the production of capsules with a small diameter (0.8-1 mm) an electrostatic generator was applied. To enhance of the capsule stability, the fermentation was performed in modified MRS medium without phospates and with addition of CaCl2 . As a result of the immobilization the bacteriocin production was increased with approximately 50 %, compared with the batch fermentation with free cells. The immobilized cells could be reuses up to three times after filtration and re-suspension in new medium.

 
© Copyright 2002 National Bank for Industrial Microorganisms and Cell Cultures - Bulgaria
Alternative site location: http://www.nbimcc.org/JCC/NBIMCC-JCC.htm

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