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NEW CUCUMOVIRUS ON BEANS IN BULGARIA – AN ATTEMPT FOR CHARACTERIZATION Kostova, Dimitrina; Tsorlianis, Stefanos; Yordanova, Angela; Vittoria, Lisa & Vaira, Anna-Maria Abstract A virus was isolated from naturally infected bean plants, grown in different areas in Bulgaria. The investigated isolates were characterized using electron microscopy, serology, biological tests, electrophoretic analysis and reverse transcription-poly-merase chain reaction (RT-PCR). Isometric particles about 30 nm in diameter were observed in crude sap preparations. The tested isolates were serologically distantly related to cucumber mosaic virus (CMV) but in ELISA experiments and immunoelectrophoresis they demonstrated a close serological relationship with a cucumovirus from China named Ch 39. The Bulgarian isolates were identified as a cucumovirus Ch 39-BG. The electrophoretic analysis of this virus showed the presence of two protein bands. CMV primers amplified an unspecific DNA fragment of 500 bp. All the performed investigations suggested the presence of a cucumovirus closer to CMV though different from the known CMV strains. Keywords cucumovirus, bean, electron microscopy, ELISA, immunoelectrophoresis, SDS-PAGE, RT-PCR.

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