search
for
 About Bioline  All Journals  Testimonials  Membership  News


Journal of Culture Collections
National Bank for Industrial Microorganisms and Cell Cultures
ISSN: 1310-8360
Vol. 6, No. 1, 2009, pp. 52-60
Bioline Code: cc09007
Full paper language: English
Document type: Research Article
Document available free of charge

Journal of Culture Collections, Vol. 6, No. 1, 2009, pp. 52-60

 bg Сравнително Изследване Върху Характеризирането На Три Стафилококови Изолата От Различен Произход
Nandy, Poulomi; Roy, Soumen; Thakur, Ashoke Ranjan & Chaudhuri, Shaon Ray


Два щама Staphylococcus check for this species in other resources , изолирани от проби на урина и слюнка, са срав- нени с лабораторен щам на Staphylococcus. Тъй като и трите изолата рас- тат на средата специфична за S. aureus, тяхното родство е изследвано по морфологични, биохимични и молекулярни характеристики. Вариацията в свойствата на изолатите, сочеща за различна идентичност, е потвърдена чрез филогенетичен анализ на база 16S рРНК. Установено е, че изолат А от урина е тясно родствен на S. haemolyticus, докато изолат Р от слюнка е S. aureus, а изолат S от колекцията NICED е най-близък до S. epidermidis. Следователно средата Hicrome aureus agar (Himedia M1468) не е специфична за вид S. aureus, а може да диференцира само до ниво род. Антибиотичният профил на щамовете е изпитан чрез спектър от 18 антибиотици. Лецити- назната активност се запазва само при прясно изолирани щамове и напълно се губи при последващо субкултивиране.

 
 en Comparative Study on Characterization of Three Staphylococcal Isolates from Varied Origin
Nandy, Poulomi; Roy, Soumen; Thakur, Ashoke Ranjan & Chaudhuri, Shaon Ray

Abstract

Two strains of Staphylococcus check for this species in other resources isolated from urine and sputum samples were compared with a laboratory strain of Staphylococcus. Since all three grew on the same media specific for S. aureus, they were studied for their relatedness by morphological, biochemical and molecular characterization. Variation in properties among the isolates pointed towards their different identity which was confirmed through 16S rRNA gene sequence based phylogenetic analysis. The isolate A from urine was found to be closely related to S. haemolyticus, while isolate P from sputum was S. aureus and isolate S from NICED culture collection was closest to S. epidermidis. Thus the Hicrome aureus agar (Himedia M1468) was not specific for the species S. aureus and could only screen at genus level. The antibiotic profile of the strains was evaluated using a spectrum of 18 antibiotics. The lecithinase activity was retained only in freshly isolated strains and totally ceased in subsequent subculturing.

Keywords
Staphylococcus aureus, lecithinase, strain characterization, molecular analysis

 
© Copyright © 2009 - National Bank for Industrial Microorganisms and Cell Cultures - Bulgaria
Alternative site location: http://www.nbimcc.org/JCC/NBIMCC-JCC.htm

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2024, Site last up-dated on 01-Sep-2022.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Google Cloud Platform, GCP, Brazil