Four strains of Gram-negative, rod-shaped, moderately halophilic bacteria,
Group A, and eleven strains of strictly aerobic, extremely halophilic rods (10 strains,
Group B) and coccoid (1 strain, Group C) were isolated from fish sauce fermentation
(nam-pla) in Thailand. The 16S rRNA gene sequence analyses of the representative
strains indicated that DS26-2 (Group A), HDS2-5 (Group B), and HRF6 (Group C),
were closely related to
Chromohalobacter salexigens
KCTC 12941
T,
Halobacteirum salinarum
JCM 8978
T, and
Halococcus saccharolyticus
JCM 8878
T with 99.3, 99.9,
and 99.0 % similarity, respectively. Group A strains were identified as
C. salexigens,
Group B as
H. salinarum, and Group C strain was
H. saccharolyticus based on their
DNA-DNA relatedness. Group A strains grew in 3–25 % (w/v) NaCl. Ubiquinone with
nine isoprene units (Q-9) was a major component. The DNA G+C contents ranged
from 63.1 to 64.2 mol %. Group B and Group C strains grew optimally in the presence
of 25-30 % NaCl. The tested strains of Group B contained major menaquinone
with eight isoprene units (MK-8). DNA G+C contents ranged from 63.3 to 64.7 mol %.
Group C strain had MK-8(H
2) as a predominant menaquinone. The DNA G+C content
was 63.2 mol %.
