Cryopreservation of Bifidobacteria and Bacteriophages in Belarusian Collection of Non-Pathogenic Microorganisms

The paper deals with cryopreservation of bifidobacteria and bacteriophages. Effect of high rates of cooling on survival ability and physiological activity of bifidobacteria is evaluated. The possibility to use rapid freezing for cryopreservation of stock Pseudomonas phage cultures is examined. The study indicates that rapid cooling kinetics appeared to lead to minimal losses in viability and acidification activity of bifidobacteria. Media traditionally used for bifidobacteria cultivation proved to have good cryoprotective properties and could be effective freezing media for preservation of these organisms. The study reveals that cryopreservation with high rates of freezing ensures high survival and maintenance of lytic ability of Pseudomonas bacteriophages. 10 % glycerol and 5 % DMSO showed good results for preservation of this group of phages.