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Ciência Florestal
Centro de Pesquisas Florestais - CEPEF, Departamento de Ciências Florestais - DCFL, Programa de Pós Graduação em Engenharia Florestal - PPGEF
ISSN: 0103-9954
EISSN: 0103-9954
Vol. 22, No. 3, 2012, pp. 605-616
Bioline Code: cf12056
Full paper language: English
Document type: Note
Document available free of charge

Ciência Florestal, Vol. 22, No. 3, 2012, pp. 605-616

 pt ESTABELECIMENTO IN VITRO DE CLONES HÍBRIDOS DE Eucalyptus globulus check for this species in other resources
Borges, Silvano Rodrigues; Xavier, Aloisio; de Oliveira, Leandro Silva; Lopes, Aline Pontes; Otoni, Wagner Campos; Takahashi, Elizabete Keiko & de Melo, Lucas Amaral

Resumo

O presente estudo teve como objetivos avaliar o comportamento de clones de Eucalyptus grandis check for this species in other resources W.Hill ex Maiden x Eucalyptus globulus check for this species in other resources Labill e Eucalyptus urophylla check for this species in other resources S.T.Blake x Eucalyptus globulus Labill e o efeito de diferentes introduções in vitro (aos 30, 90 e 150 dias após a poda do ápice das minicepas) na fase de estabelecimento in vitro. As minicepas, fornecedoras dos explantes para a introdução in vitro, foram conduzidas em minijardim clonal semi-hidropônico. Segmentos nodais de 21 clones de Eucalyptus urophylla x Eucalyptus globulus e 8 clones de Eucalyptus grandis x Eucalyptus globulus foram coletados, desinfestados e inoculados em meio de cultura MS, suplementado com 0,5 mg L-1 de BAP e 0,1 mg L-1 de ANA. Após 30 dias em cultura, foram feitas as avaliações e concluiu-se que houve variação entre clones, bem como entre introduções in vitro, para as variáveis estudadas, sendo poss ível o estabelecimento in vitro da maioria dos clones.

Palavras-chave
Micropropagação; introdução in vitro; clonagem

 
 en IN VITRO ESTABLISHMENT OF Eucalyptus globulus check for this species in other resources HYBRID CLONES
Borges, Silvano Rodrigues; Xavier, Aloisio; de Oliveira, Leandro Silva; Lopes, Aline Pontes; Otoni, Wagner Campos; Takahashi, Elizabete Keiko & de Melo, Lucas Amaral

Abstract

The present study aimed to evaluate the behavior of Eucalyptus grandis check for this species in other resources x Eucalyptus globulus check for this species in other resources and Eucalyptus urophylla check for this species in other resources x Eucalyptus globulus clones and the effect of different in vitro insertions (at 30, 90 and 150 days after pruning the donor plants) in the in vitro establishment phase. The mini-stump plants, explant providers for the in vitro introductions, were cultivated under an indoor semi-hydroponic clonal mini-hedging. Nodal segment explants of 21 Eucalyptus urophylla x Eucalyptus globulus clones and eight Eucalyptus grandis x Eucalyptus globulus clones were harvested, disinfected and inoculated in an MS culture medium, supplemented with 0.5 mg L-1 of BAP and 0.1 mg L-1 of NAA. After 30 days in culture, evaluations were made and it was concluded that there were variations among the clones, as well as in vitro introductions, for the studied variables, where the in vitro establishment was possible for most clones.

Keywords
Micropropagation; in vitro introduction; cloning

 
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