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Ciência Florestal
Centro de Pesquisas Florestais - CEPEF, Departamento de Ciências Florestais - DCFL, Programa de Pós Graduação em Engenharia Florestal - PPGEF
ISSN: 0103-9954
EISSN: 1980-5098
Vol. 26, No. 2, 2016, pp. 463-473
Bioline Code: cf16041
Full paper language: Portuguese
Document type: Research Article
Document available free of charge

Ciência Florestal, Vol. 26, No. 2, 2016, pp. 463-473

 en MORPHOLOGICAL, MOLECULAR AND PATOGENICITY CHARACTERIZATION OF Fusarium acuminatum check for this species in other resources and Fusarium verticillioides TO Cordia americana check for this species in other resources SEEDS
Walker, Clair; Maciel, Caciara Gonzatto; Milanesi, Paola Mendes; Muniz, Marlove Fátima Brião; Mezzomo, Ricardo & Pollet, Camila Schultz

Abstract

Cordia americana check for this species in other resources (L.) Gottschling & J. S. Mill. (guajuvira) is a forest species widely distributed in Brazil and represents environmental and economic importance due to the quality of its timber and use in reforestation. In order to evaluate the pathogenicity of Fusarium check for this species in other resources sp. associated with seeds, characterizing the isolates morphologically and molecularly in order to identify them on the species level, was obtained four fungal isolates that were inoculated in seeds to demonstrate their pathogenicity. The morphological characterization was performed using an identification key for the genus Fusarium and, for the molecular identification, genomic regions Internal Transcribed Spacer (ITS) were sequenced with its elongation factor 1 alpha (TEF1-α). The results of the molecular identification agreed with the morphological characterization and allowed to identify the species Fusarium acuminatum check for this species in other resources and Fusarium verticillioides, both being pathogenic to Cordia americana, causing damping-off in pre and post emergence, and rotting of roots and necrosis of hypocotyl.

Keywords
damping-off; Guajuvira; ITS; TEF1-α

 
 pt CARACTERIZAÇÃO MORFOLÓGICA, MOLECULAR E PATOGENICIDADE DE Fusarium acuminatum check for this species in other resources E Fusarium verticillioides A Cordia americana check for this species in other resources
Walker, Clair; Maciel, Caciara Gonzatto; Milanesi, Paola Mendes; Muniz, Marlove Fátima Brião; Mezzomo, Ricardo & Pollet, Camila Schultz

Resumo

Cordia americana check for this species in other resources (L.) Gottschling & J. S. Mill. (guajuvira) é uma espécie florestal amplamente distribuída no território brasileiro e que apresenta importância econômica e ambiental devido à qualidade de sua madeira e utilização em reflorestamentos. Com o objetivo de avaliar a patogenicidade de Fusarium check for this species in other resources sp. associado às sementes, foram caracterizados, morfológica e molecularmente, quatro isolados do fungo que foram inoculados nas sementes de guajuvira. A caracterização morfológica foi realizada com o auxílio de uma chave de identificação para o gênero Fusarium e para a identificação molecular foram sequenciadas as regiões genômicas Internal Transcribed Spacer (ITS) e Fator de elongação 1 - alpha (TEF1-α). Os resultados do sequenciamento de DNA concordaram com os da caracterização morfológica e permitiram identificar as espécies Fusarium acuminatum check for this species in other resources e Fusarium verticillioides. No teste de patogenicidade, verificou-se que ambas as espécies do fungo foram patogênicas a Cordia americana, causando tombamento em pré e pósemergência, além de apodrecimento de raízes e necrose no hipocótilo.

Palavras-chave
tombamento de plântulas; guajuvira; ITS; TEF1-α

 
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Alternative site location: http://cascavel.ufsm.br/revistas/ojs-2.2.2/index.php/cienciaflorestal/index

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