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Revista Científica UDO Agrícola
Universidad de Oriente Press
ISSN: 1317-9152
Vol. 12, No. 3, 2012, pp. 609-616
Bioline Code: cg12069
Full paper language: English
Document type: Research Article
Document available free of charge

Revista Científica UDO Agrícola, Vol. 12, No. 3, 2012, pp. 609-616

 en Integrated control of white grubs Phyllophaga vetula check for this species in other resources Horn (Coleoptera: Melolonthidae) with entomopathogenic agents in Oaxaca, Mexico
VEGA, Jaime RUIZ; BOLAÑOS, Teodulfo AQUINO; RIVERA, María Eugenia SILVA & PABLO, Sergio GIRÓN

Abstract

In Mexico, chemical control of white grubs is frequently used; therefore, it is necessary to find alternative control methods. An entomopathogenic fungi and nematodes were evaluated against larvae of Phyllophaga vetula check for this species in other resources Horn under semicontrolled and field conditions. The semi-controlled experiment revealed that maize ( Zea mays check for this species in other resources L.) in the V2 development stage tolerated only one grub per plant, while four larvae per plant were necessary to cause a significant damage to maize plants in V4 stage. In another experiment, it was found that Metarhizium anisopliae check for this species in other resources Metsch. (1 x 105 spores/cm2) and the combination of M. anisopliae (1 x 105 spores/ cm2) + Heterorhabditis bacteriophora check for this species in other resources Poinar (25 Infective Juveniles (IJ)/cm2) achieved an 87.5 % control in 20 days. Besides, it was found that Steinernema carpocapsae check for this species in other resources Weiser (ALL strain) achieved a similar control rate as the insecticide, showing that it was possible to get a significant control (P≤0,05) with only 10 IJs/cm2. In the field experiments the most outstanding combination was M. anisopliae at 2x108 spores/plant + S. carpocapsae at a dose of 1500 IJs/plant.

Keywords
Metarhizium anisopliae, Heterorhabditis bacteriophora, Steinernema feltiae, Steinernema carpocapsae

 
 es Control integrado de la gallina ciega Phyllophaga vetula check for this species in other resources Horn (Coleoptera: Melolonthidae) con agentes entomomopatógenos en Oaxaca, México
VEGA, Jaime RUIZ; BOLAÑOS, Teodulfo AQUINO; RIVERA, María Eugenia SILVA & PABLO, Sergio GIRÓN

Resumen

En México el combate de gallinas ciegas se realiza utilizando generalmente insecticidas químicos, por lo que es necesario encontrar métodos alternativos de control. Con este propósito, se evaluaron un hongo entomopatógeno y nematodos para el control de larvas de Phyllophaga vetula check for this species in other resources Horn en dos tipos de experimentos: semicontrolados y de campo. En experimentos semicontrolados se encontró que se requiere de una larva de segundo estadio/planta para afectar significativamente (p ≤ 0,05) a plantas de maíz ( Zea mays check for this species in other resources L.) en V2, mientras que en la etapa V4 se requieren de cuatro larvas por planta. Se encontró que el hongo entomopatógeno Metarhizium anisopliae check for this species in other resources Metsch (1x105 esporas/cm2) y la combinación de M. anisopliae (1x105 esporas/cm2) y el nematodo entomopatógeno Heterorhabditis bacteriophora check for this species in other resources Poinar (25 Juveniles Infectivos (JI)/cm2) requirió de 20 días para lograr un 87,5 % de control. Además se encontró que Steinernema carpocapsae check for this species in other resources Weiser (cepa ALL) fue el tratamiento con el mayor porcentaje de control después del insecticida, mostrando que con una dosis de 10 JI/cm2 es posible obtener un control significativo (p ≤ 0,05). Los experimentos de campo mostraron que la combinación de entomopatógenos más sobresaliente fue M. anisopliae a una dosis de 2x108 esporas/planta + S. carpocapsae a una dosis de 1500 JI/planta.

Palabras-clave
Metarhizium anisopliae, Heterorhabditis bacteriophora, Steinernema feltiae, Steinernema carpocapsae

 
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