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Revista Científica UDO Agrícola
Universidad de Oriente Press
ISSN: 1317-9152
Vol. 12, No. 4, 2012, pp. 839-844
Bioline Code: cg12095
Full paper language: Spanish
Document type: Research Article
Document available free of charge

Revista Científica UDO Agrícola, Vol. 12, No. 4, 2012, pp. 839-844

 es Actividad antifúngica in vitro e in vivo del extracto acuoso de hojas de Parthenium hysterophorus check for this species in other resources L. sobre Pyricularia grisea check for this species in other resources Sacc.
RODRÍGUEZ PEDROSO, Aida Tania; RAMÍREZ ARREBATO, Miguel Ángel; CÁRDENAS TRAVIESO, Regla María; RIVERO GONZÁLEZ, Deyanira; TRIANA, Ariel Cruz & BAUTISTA BAÑOS, Silvia

Resumen

Las plantas sintetizan metabolitos o compuestos químicos con actividad antimicrobiana, los cuales le confieren una protección natural contra hongos, bacterias e insectos. En este trabajo se obtuvo el extracto acuoso de hojas de Parthenium hysterophorus check for this species in other resources L. con el objetivo de evaluar su actividad antifúngica in vitro contra el hongo Pyricularia grisea check for this species in other resources Sacc, responsable de la enfermedad más importante en el arroz ( Oryza sativa check for this species in other resources , L.), evaluando el porcentaje de inhibición del crecimiento micelial en el tiempo. Además se evaluó in vivo el efecto protector del extracto contra P. grisea Sacc. en plántulas de arroz. Se realizó el análisis químico cualitativo del extracto. Se encontró que el extracto tenía elevada actividad antifúngica sobre el aislamiento PGS-05. Con una concentración de 10% de extracto la inhibición del hongo fue total, además de tener actividad biocida. En el ensayo in vivo se observó que los diferentes cultivares de arroz a las cuales se aplicó el extracto mostraron menor afectación por P. grisea Sacc. comparado con los tratamientos testigos. Se determinó que el extracto contiene metabolitos con efecto antimicrobiano como: taninos, fenoles, flavonoides y triterpenos. Por lo que, el extracto acuoso de P. hysterophorus L. podría ser utilizado en el control de la piriculariosis en el cultivo del arroz.

Palabras-clave
Pyricularia grisea; taninos; metabolitos secundarios; Parthenium hysterophorus; piriculariosis

 
 en in vitro and in vivo antifungal activity of the aqueous extract of Parthenium hysterophorus check for this species in other resources L. against Pyricularia grisea check for this species in other resources Sacc.
RODRÍGUEZ PEDROSO, Aida Tania; RAMÍREZ ARREBATO, Miguel Ángel; CÁRDENAS TRAVIESO, Regla María; RIVERO GONZÁLEZ, Deyanira; TRIANA, Ariel Cruz & BAUTISTA BAÑOS, Silvia

Abstract

Plants synthetize metabolites or chemical compound with antimicrobial activity, which give them natural protection against fungus, bacteria and insects. In this work, an aqueous extract from Parthenium hysterophorus check for this species in other resources L. leaves was obtained with the objective of testing its in vitro antifungal activity against the fungus Pyricularia grisea check for this species in other resources Sacc. which cause the most important disease of rice ( Oryza sativa check for this species in other resources L.) evaluating the inhibition percent of mycelial growth through time. Also, the protective effect of the extract was evaluated in vivo against P. grisea Sacc. in rice seedlings. A qualitative chemical analysis of the extract was carried out. It was found that the extract had high antifungal activity against the isolate PGS-05. A concentration of 10% of the extract, the inhibition of fungus was complete, besides it had biocidal activity. It was observed in in vivo test that the rice cultivars which received the extract showed less damage by P. grisea Sacc. as compared with control treatments. It was found that the extract contained antimicrobial metabolites such as tannins, phenols, flavonoids and triterpenes. Therefore, the aqueous extract of P. hysterophorus L. leaves could be used in piriculariosis control in rice cultivation.

Keywords
Pyricularia grisea; tannins; secondary metabolites; Parthenium hysterophorus; piriculariosis

 
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