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Chilean Journal of Agricultural Research
Instituto de Investigaciones Agropecuarias, INIA
ISSN: 0718-5820
EISSN: 0718-5839
Vol. 69, No. 4, 2009, pp. 526-533
Bioline Code: cj09063
Full paper language: English
Document type: Research Article
Document available free of charge

Chilean Journal of Agricultural Research, Vol. 69, No. 4, 2009, pp. 526-533

 es Identificación y caracterización biológica de cepas de bacillus con actividad inhibitoria contra Macrophomina phaseolina check for this species in other resources (Tassi) Goid
Gacitúa, Sandra A.; Valiente, Carolina F.; Díaz, Katy P.; Hernández, José C.; Uribe, Matilde M. & Sanfuentes, Eugenio V.

Resumen

El hongo Macrophomina phaseolina check for this species in other resources (Tassi) Goid., agente causal de la pudrición carbonosa de la raíz, es considerado uno de los patógenos más importantes en viveros forestales. Estudios recientes realizados en Chile comprobaron la capacidad antagónica de diversas cepas bacterianas contra M. phaseolina, tanto en ensayos in vitro como de campo. Los objetivos de este estudio fueron identificar las cepas de Bacillus check for this species in other resources y establecer los mecanismos de antagonismo contra M. phaseolina. La identificación de las cepas se realizó mediante kit bioquímico bioMérieux. Los ensayos fueron realizados in vitro y consistieron en determinar la producción de metabolitos volátiles, sideróforos y el efecto de diferentes temperaturas de cultivo (20, 25, 30 y 35 °C) en la producción de metabolitos difusibles e inhibición del crecimiento micelial del patógeno. Las cepas fueron identificadas como Bacillus subtilis check for this species in other resources IX 007, B. amyloliquefaciens VII 015 y VIII 016, B. pumilus IX 030 y B. stearothermophilus TM 008. No fue posible la identificación de la cepa Gram (-) VI 009. En todas las temperaturas ensayadas, las cepas inhibieron M. phaseolina, alcanzando hasta 75% de reducción del crecimiento micelial (B. subtilis IX 007 a 25 °C). Las cepas mostraron como mecanismos de antagonismo la producción de metabolitos volátiles y de sideróforos.

Palabras-clave
control biológico, Bacillus, Pinus radiata, viveros forestales

 
 en Identification and biological characterization of isolates with activity inhibitive against Macrophomina phaseolina check for this species in other resources (Tassi) Goid
Gacitúa, Sandra A.; Valiente, Carolina F.; Díaz, Katy P.; Hernández, José C.; Uribe, Matilde M. & Sanfuentes, Eugenio V.

Abstract

The Macrophomina phaseolina check for this species in other resources (Tassi) Goid fungus, causal agent of charcoal root rot, is considered one of the most important pathogens in forest nurseries. Recent studies in Chile have verified the antagonistic capacity of diverse bacterial isolates against M. phaseolina in vitro as well as in field testing. The objectives of this study were to identify Bacillus check for this species in other resources isolates and establish the mechanisms of antagonism against M. phaseolina. Isolates were identified with the bioMérieux biochemical kit. Tests were done in vitro and consisted in determining the production of volatile metabolites, siderophores, and the effect of different culture temperatures (20, 25, 30, and 35 °C) on the production of diffusible metabolites and inhibition of mycelial growth of the pathogen. The isolates were identified as Bacillus subtilis check for this species in other resources IX 007, B. amyloliquefaciens VII 015 and VIII 016, B. pumilus IX 030, and B. stearothermophilus TM 008. It was not possible to identify the (-) Gram VI 009 isolate. At all the evaluated temperatures, isolates inhibited M. phaseolina, reaching 75% reduction of mycelial growth (B. subtilis IX 007 at 25 °C). Some isolates showed volatile metabolites and siderophore production as possible mechanisms of antagonism.

Keywords
biocontrol, Bacillus, Pinus radiata, forest nursery

 
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