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Chilean Journal of Agricultural Research
Instituto de Investigaciones Agropecuarias, INIA
ISSN: 0718-5820
EISSN: 0718-5839
Vol. 69, No. 4, 2009, pp. 534-540
Bioline Code: cj09064
Full paper language: English
Document type: Research Article
Document available free of charge

Chilean Journal of Agricultural Research, Vol. 69, No. 4, 2009, pp. 534-540

 es Selección de hongos entomopatógenos para el control de Varroa destructor check for this species in other resources (Acari: Varroidae)
Rodríguez, Marta; Gerding, Marcos & France, Andrés

Resumen

El objetivo de este trabajo fue seleccionar hongos entomopatógenos tolerantes a las temperaturas del nido de cría de las abejas ( Apis mellifera check for this species in other resources ), para ser utilizados en el control de Varroa destructor check for this species in other resources . Se evaluaron 50 aislamientos de Beauveria bassiana check for this species in other resources (Balsamo) Vuillemin y 48 de Metarhizium anisopliae check for this species in other resources (Metschn.) Sorokin a temperaturas de 30 y 35 ºC. Discos de agar de 5 mm de diámetro con micelio de colonias de cada aislamiento, se depositaron en el centro de placas Petri con medio agar Sabouraud dextrosa (ASD). Las placas fueron incubadas a 30 y 35 °C y oscuridad y diariamente se midió el radio de cada colonia. Todos los aislamientos de B. bassiana y M. anisopliae var. anisopliae presentaron una tendencia lineal a través del tiempo a temperaturas de incubación de 30 °C. A 35 °C la mayoría de los aislamientos no crecieron, excepto tres aislamientos de B. bassiana y 14 de M. anisopliae (p < 0,001). Estos aislamientos fueron seleccionados para realizar pruebas de patogenicidad sobre V. destructor, aplicando una suspensión de 107 conidias mL-1. El aislamiento más efectivo fue Qu-M845 de M. anisopliae (p = 0,0033), produjo una mortalidad de 85%. La capacidad patogénica de este aislamiento en V. destructor y su tolerancia a las condiciones ambientales a las que sería expuesto, permite considerar esta cepa como una alternativa de control para esta plaga.

Palabras-clave
control biológico, temperatura, hongo entomopatógeno, Varroa destructor, varroasis

 
 en Selection of entomopathogenic fungi to control Varroa destructor check for this species in other resources (Acari: Varroidae)
Rodríguez, Marta; Gerding, Marcos & France, Andrés

Abstract

The aim of this work was select entomopathogenic fungi tolerant to temperatures inside the brood area of honey bees ( Apis mellifera check for this species in other resources ) for to control Varroa destructor check for this species in other resources . For this purpose, 50 Beauveria bassiana check for this species in other resources (Balsamo) Vuillemin and 48 Metarhizium anisopliae check for this species in other resources (Metschn.) Sorokin isolates were evaluated at 30 and 35 ºC. For each isolate, colony discs of 5 mm with mycelium were placed in the center of a Petri dish with Sabouraud dextrose agar (SDA) medium. The dishes were incubated at 30 and 35 °C, without light. Radial growth of each colony was measured daily. All the B. bassiana and M. anisopliae isolates presented a lineal growth rate at a temperature of 30 ºC. However, at 35 ºC, most of the isolates did not grow, except three B. bassiana and 14 M. anisopliae isolates (P < 0.001). These isolates were selected to evaluate the pathogenicity on V. destructor by applying a suspension of 107 conidia mL-1. The most effective isolate was M. anisopliae Qu-M845 (P = 0.0033) which produced 85% mortality. The pathogenic capacity of this isolate on V. destructor and its tolerance to hive conditions allows us to consider this strain as an alternative to control this pest.

Keywords
biological control, entomopathogenic fungi, Varroa destructor, varroasis, entomopathology

 
© Copyright 2009 Chilean Journal of Agricultural Research.
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