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Chilean Journal of Agricultural Research
Instituto de Investigaciones Agropecuarias, INIA
ISSN: 0718-5820
EISSN: 0718-5839
Vol. 71, No. 1, 2011, pp. 39-44
Bioline Code: cj11005
Full paper language: English
Document type: Research Article
Document available free of charge

Chilean Journal of Agricultural Research, Vol. 71, No. 1, 2011, pp. 39-44

 es Sensibilidad de Aislados Chilenos de Venturia inaequalis a Difenoconazole, Fenarimol, Mancozeb y Pyrimethanil.
Henríquez S., Jose Luis; Sarmiento V., Oliver & Alarcón C., Paula

Resumen

La sarna del manzano (Venturia inaequalis) es la principal enfermedad del cultivo en Chile y es controlada principalmente con el uso de fungicidas. Se realizaron pruebas de sensibilidad a los fungicidas difenoconazole, fenarimol, mancozeb y pyrimethanil en aislados monoconidiales de cepas silvestres de V. inaequalis con el fin de elaborar curvas basales de sensibilidad y compararlas con la sensibilidad de una colección de aislados monoconidiales provenientes de siete huertos comerciales sin evidencias de resistencia práctica. Se realizaron pruebas in vitro donde la sensibilidad se midió como la inhibición del crecimiento miceliar (difenoconazole, fenarimol y pyrimethanil) o la inhibición de la germinación de conidias (mancozeb). La población de huertos comerciales estudiada presentó una menor sensibilidad a los fungicidas difenoconazole, fenarimol y mancozeb con valores de factor de resistencia 4,7; 5,8; y 2,1 para difenoconazole, fenarimol y mancozeb, respectivamente. La mayor diferencia de sensibilidad entre la población silvestre y la de huerto se obtuvo con feranimol, fungicida para el cual se observó el mayor cambio hacia una menor sensibilidad. Se proponen dosis discriminatorias de 0,04; 1,0; 0,6; y 0,2 µg mL-1 para difenoconazole, fenarimol, mancozeb y pyrimethanil, respectivamente.

Palabras-clave
manzano, sarna del manzano, fungicidas, fungicidas IBE, ditiocarbamatos, anilinopirimidinas.

 
 en Sensitivity of Venturia inaequalis Chilean Isolates to Difenoconazole, Fenarimol, Mancozeb, and Pyrimethanil
Henríquez S., Jose Luis; Sarmiento V., Oliver & Alarcón C., Paula

Abstract

Apple scab (Venturia inaequalis) is the most important disease in Chilean apple (Malus domestica Borkh.) orchards where fungicide management is the main tool to control disease. Sensitivity tests to difenoconazole, fenarimol, mancozeb, and pyrimethanil fungicides were conducted on V. inaequalis wild isolates to construct local baseline sensitivity distributions, and compare them with a collection of isolates from seven different commercial orchards with no evidence of practical resistance. in vitro assays were conducted and sensitivity was measured as inhibition of mycelial growth (difenoconazole, fenarimol, pyrimethanil) or conidial germination (mancozeb). Departure from the baseline distribution to less sensitivity was found for difenoconazole, fenarimol, and mancozeb with resistance factors of 4.7, 5.8, and 2.1, respectively. The highest difference between baseline and orchard populations was observed for fenarimol which showed the highest shift to less sensitivity. Discriminatory doses of 0.04, 1.0, 0.6, and 0.2 µg mL-1 are proposed for in vitro monitoring of sensitivity to difenoconazole, fenarimol, mancozeb, and pyrimethanil, respectively.

Keywords
Apple, apple scab, fungicides, DMI fungicides, dithiocarbamates, anilinopyrimidines.

 
© Copyright 2010 Chilean Journal of Agricultural Research.
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