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Chilean Journal of Agricultural Research
Instituto de Investigaciones Agropecuarias, INIA
ISSN: 0718-5820
EISSN: 0718-5839
Vol. 71, No. 2, 2011, pp. 249-257
Bioline Code: cj11030
Full paper language: English
Document type: Research Article
Document available free of charge

Chilean Journal of Agricultural Research, Vol. 71, No. 2, 2011, pp. 249-257

 es Digestibilidad mediante pepsina-celulasa de ensilajes de pradera: efectos del tipo de pradera, estado demadurez y variaciones en el método enzimático
Barchiesi-Ferrari, Claudia; Alomar, Daniel & Miranda, Horacio

Resumen

La digestibilidad enzimática in vitro ha sido estudiada parapredecir el valor energético de forrajes para rumiantes, aunque el tipo de forraje y los detalles metodológicos han afectado los resultados obtenidos. Este trabajo pretende evaluar los efectos de la concentración de celulasa (0,75; 1,0 y 6,25 g L-1), tiempo de incubación (24 ó 48 h) y tipo de lavado final (agua o acetona) del residuo, sobre la digestibilidad in vitro de la materia seca (DMD), materia orgánica (OMD) y el contenido de materia orgánica digestible en la materia seca, o valor D (D value) de ensilajes hechos con tres estados de madurez, de diferentes praderas: a) pradera permanente ( Dactylis glomerata check for this species in other resources , Lolium perenne check for this species in other resources , Bromus catharticus check for this species in other resources Vahl var. catharticus, Trifolium repens check for this species in other resources y Holcus lanatus check for this species in other resources ); b)ballica Italiana ( Lolium multiflorum check for this species in other resources Lam. cv. Tama); c) avena ( Avena sativa check for this species in other resources L.); y d) pradera mixta (L. perenne-T. repens). Además, se ajustaron regresiones entre resultados con celulasas y digestibilidad in vitro con licor ruminal. Un incremento en la concentración de enzima, mayor tiempo de incubación y lavado con acetona incrementaron (P < 0,05) la DMD, OMD y valor D. Se detectó interacción (P < 0,001) entre tipo de forraje, estado de madurez y tiempo de incubación y también (P < 0,05) entre estado de madurez y concentración enzimática, para valor D, DMD y OMD. El tipo de pradera y estado fenológico afectaron la capacidad del método enzimático para predecir la digestibilidad in vitro obtenida con licor ruminal. Por tanto, se debería desarrollar diferentes ecuaciones de predicción para cualquier combinación de los factores antes mencionados. El ajuste de esta metodología con valores in vivo ayudaría a mejorar las predicciones de valores de digestibilidad.

Palabras-clave
incubación con celulasas, digestibilidad enzimática, digestibilidad in vitro.

 
 en Pepsin-Cellulase Digestibility of Pasture Silages: Effects Of Pasture Type, Maturity Stage, and Variations in the enzymatic Method
Barchiesi-Ferrari, Claudia; Alomar, Daniel & Miranda, Horacio

Abstract

Enzymatic in vitro digestibility has been studied as a method to predict energy values of forages for ruminants, although results have been affected by type of forage and methodological details of the technique. This work was performed to evaluate the effects of cellulase concentration (0.75, 1.0 and 6.25 g L-1), incubation time (24 or 48 h) and type of final washing of the residue (water or acetone) on the in vitro digestibility of the dry matter (DMD), organic matter (OMD) and content of digestible organic matter in the DM (D value) of silages made at three maturity stages from three types of pastures: a) permanent pasture ( Dactylis glomerata check for this species in other resources , Lolium perenne check for this species in other resources , Bromus catharticus check for this species in other resources Vahl var. catharticus, Trifolium repens check for this species in other resources and Holcus lanatus check for this species in other resources ); b) Italian ryegrass ley ( Lolium multiflorum check for this species in other resources Lam. cv. Tama); c) oats ( Avena sativa check for this species in other resources L.) and d) mixed pasture (L. perenne-T. repens). Regression equations among cellulase results and in vitro values obtained with rumen fluid were also developed. Higher enzyme concentration, longer incubation time and final washing with acetone, resulted in a significant (P < 0.05) increase in DMD, OMD and D value. A strong interaction (P < 0.001) among forage type, maturity stage and incubation time was observed. Interactions between stage and enzyme concentration were also apparent (P < 0.05) for D value, DMD and OMD. Type of pasture and maturity affected the performance of the enzymatic method to predict in vitro digestibility obtained with rumen fluid. Therefore, different prediction equations should be developed for any combination of the above mentioned factors. Adjusting this methodology with in vivo data is encouraged to improve prediction of digestibility values.

Keywords
Cellulase incubation, enzymatic digestibility, in vitro digestibility

 
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