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Chilean Journal of Agricultural Research
Instituto de Investigaciones Agropecuarias, INIA
ISSN: 0718-5820
EISSN: 0718-5839
Vol. 71, No. 3, 2011, pp. 376-382
Bioline Code: cj11045
Full paper language: English
Document type: Research Article
Document available free of charge

Chilean Journal of Agricultural Research, Vol. 71, No. 3, 2011, pp. 376-382

 en IN VITRO PROPAGATION OF CEDAR ( Cedrela odorata check for this species in other resources L.) FROM JUVENILE SHOOTS
García-Gonzáles, Rolando; Delgado, Miladys; González, Yailín; González, Aníbal; Garriga, Miguel; Caligari, Peter D.S.; Carrasco, Basilio & Quiroz, Karla

Abstract

Cedrela odorata check for this species in other resources L. is one of the most important timber species currently traded in the Caribbean and Central America; however, it has been intensively exploited. In vitro techniques and clonal propagation can help to develop new plantations and assist in establishing improvement programs for this species. The aim of this study was to develop a protocol to establish in vitro conditions and to micropropagate this species from nodal explants from juvenile cuttings taken from field trees. Disinfection of node explants with 5% propiconazole CE 25 during 3 min resulted in 100% explant disinfection and 60% morphogenic response on those established explants. Shoot development was optimized by cultivating in vitro node explants in Murashige and Skoog basal medium supplemented with 2 mg L-1 6-bencilaminopurine and 3 mg L-1 naphthaleneacetic acid. This medium resulted in 100% shoot development from the in vitro node explants with a 3.93 cm mean height. Rooting was also stimulated 6 wk after individualization of the regenerated plants on the same micropropagation medium with a mean of 3.9 roots per plant. In vitro plants did not show morphologic differences when compared to ex vitro seeds.

Keywords
Organogenesis, in vitro propagation, Cedrela odorata, in vitro rooting, plant growth regulators

 
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