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Chilean Journal of Agricultural Research
Instituto de Investigaciones Agropecuarias, INIA
ISSN: 0718-5820
EISSN: 0718-5839
Vol. 71, No. 4, 2011, pp. 542-548
Bioline Code: cj11069
Full paper language: English
Document type: Research Article
Document available free of charge

Chilean Journal of Agricultural Research, Vol. 71, No. 4, 2011, pp. 542-548

 en ENDOGENOUS QUANTIFICATION OF ABSCISIC ACID AND INDOLE-3- ACETIC ACID IN SOMATIC AND ZIGOTIC EMBRYOS OF Nothofagus alpina check for this species in other resources (Poepp. & Endl.) Oerst.
Riquelme, Pricila Cartes; Leal, Darcy Ríos; Carrillo, Kátia Sáez; Moraga, Matilde Uribe; Aguilar, Sofía Valenzuela; Bolus, Stephen Joseph & Olate, Manuel Sánchez

Abstract

Abscisic acid (ABA) and indole-3-acetic acid (IAA) participate in the propagation of plants by somatic embryogenesis, causing polar structural differentiation of the embryo. The goal of the assay was to compare endogenous levels of ABA and IAA between somatic embryos (SE) and zygotic embryos (ZE) of Nothofagus alpina check for this species in other resources (Poepp. & Endl.) Oerst. In this study, a somatic embryo maturation assay involving the addition of varying concentrations of exogenous ABA was performed on cotyledonary-stage of N. alpina. Furthermore, the endogenous levels of ABA and IAA were quantified in the immature ZE, the mature ZE, and the embryonic axis of a mature embryo of N. alpina. The current study utilized high performance liquid chromatography (HPLC) for quantification. The maturation treatments performed did not present significant differences in the endogenous ABA levels in SE. However, significant differences did exist in levels of ABA and IAA between SE submitted to the different maturation treatments and mature ZE of N. alpina. The application of exogenous ABA to the culture medium increased endogenous ABA levels, therefore, increasing the number of germinated somatic embryos. Thus, the plant conversion process was also successfully completed in somatic embryos of N. alpina.

Keywords
Somatic embryogenesis, maturation, germination, HPLC

 
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