search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Chilean Journal of Agricultural Research
Instituto de Investigaciones Agropecuarias, INIA
ISSN: 0718-5820
EISSN: 0718-5839
Vol. 72, No. 2, 2012, pp. 201-205
Bioline Code: cj12032
Full paper language: English
Document type: Research Article
Document available free of charge

Chilean Journal of Agricultural Research, Vol. 72, No. 2, 2012, pp. 201-205

 en SELECTION of Ento mopatho genic nemato des against Dalaca pallens check for this species in other resources (Lepidoptera: Hepialidae)
Maldonado, Alexis; Merino, Loreto & France, Andrés

Abstract

Dalaca pallens check for this species in other resources (Blanchard) is a pest with great impact on grassland production in Chile. The objective of this research was to evaluate the use of entomopathogenic nematodes (EPN) against D. pallens larvae. Twenty EPN isolates, collected along Chile, were used for nematode selection. The experimental unit consisted of five 50-mL plastic vials filled with Nothofagus dombeyi check for this species in other resources sawdust and inoculated with 40 dauers per vial. The experimental design was a completely randomized with four replicates per treatment. The results showed that isolate QU N3 ( Steinernema australe check for this species in other resources ) and QU N13 ( Steinernema unicornum check for this species in other resources ) produced the highest mortality, with 100 and 95% respectively and no differences (P < 0.001) between both. Later, these two isolates were used to calculate the lethal concentration (LC50 and LC90). Concentrations of 0, 10, 20, 30, 40, and 50 dauers per 50-mL plastic vials filled with Nothofagus sawdust were evaluated. Then, a single larva of D. pallens was added to each vial and incubated for 13 d. The results showed that LC50 and LC90 were equivalent to 14 and 39 dauers mL-1 for isolate QU N3, while for isolate QUN13 these figures were 14 and 48 dauers mL-1. Consequently, there are native isolates of EPN with the ability to control D. pallens larvae, which require future test under field conditions.

Keywords
Biological control, Steinernema australe, Steinernema unicornium, lethal concentration

 
© Copyright 2012 - Chilean Journal of Agricultural Research
Alternative site location: http://www.inia.cl

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 16-Oct-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil