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Chilean Journal of Agricultural Research
Instituto de Investigaciones Agropecuarias, INIA
ISSN: 0718-5820
EISSN: 0718-5839
Vol. 72, No. 4, 2012, pp. 507-515
Bioline Code: cj12077
Full paper language: English
Document type: Research Article
Document available free of charge

Chilean Journal of Agricultural Research, Vol. 72, No. 4, 2012, pp. 507-515

 en CHEMICAL COMPOSITION AND ANTIFUNGAL ACTIVITY OF Piper auritum check for this species in other resources KUNTH AND Piper holtonii check for this species in other resources C. DC. AGAINST PHYTOPATHOGENIC FUNGI
Pineda M., Rodrigo; Vizcaíno P., Samuel; García P., Carlos M.; Gil G., Jesús H. & Durango R., Diego L.

Abstract

The growth of fungi during pre- and postharvest of fruits may cause spoilage and result in a reduction in quality and quantity. The aim of the present work was to investigate the efficacy of four extracts (n-hexane, dichlorometane, ethyl acetate, and methanol) and the essential oil of Piper auritum check for this species in other resources Kunth and P. holtonii check for this species in other resources C. DC. on the growth inhibition of three important postharvest pathogens of fruits ( Colletotrichum acutatum check for this species in other resources , C. gloeosporioides check for this species in other resources , and Botryodiplodia theobromae check for this species in other resources ). The in vitro antifungal activity was assayed following the poisoned food technique. The results showed that both n-hexane extract (HE) as essential oil (EO) displayed a relative good control against the three pathogens, being the originating materials of P. holtonii the most actives. Mycelial growth of C. acutatum, C. gloeosporioides, and B. theobromae was significantly inhibited at 400 μg mL-1. Additionally, the chemical composition of the bioactive materials was analyzed by gas chromatography-mass spectrometry (GC-MS). Safrole (64.54/56.88%) and apiol (64.24/57.20%) were the major constituents of the EO/HE from P. auritum and P. holtonii respectively. Structural identification was also confirmed by nuclear magnetic resonance. Both compounds exhibited significant antifungal properties. It can be concluded that EOs/HEs from P. auritum and P. holtonii, and their major constituents, have interesting applications to control plant pathogenic fungi.

Keywords
Piperaceae, essential oil, apiol, Colletotrichum, Botryodiplodia

 
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