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Chilean Journal of Agricultural Research
Instituto de Investigaciones Agropecuarias, INIA
ISSN: 0718-5820
EISSN: 0718-5839
Vol. 73, No. 3, 2013, pp. 250-258
Bioline Code: cj13036
Full paper language: English
Document type: Research Article
Document available free of charge

Chilean Journal of Agricultural Research, Vol. 73, No. 3, 2013, pp. 250-258

 en Antimicrobial activity against Xanthomonas albilineans check for this species in other resources and fermentation kinetics of a lactic acid bacterium isolated from the sugar cane crop
Serna-Cock, Liliana; Camargo-Guarnizo, Andrés Felipe & Rengifo-Guerrero, Carlos Andrés

Abstract

Xanthomonas albilineans check for this species in other resources is a pathogen that causes leaf scald disease in sugarcane ( Saccharum officinarum check for this species in other resources L.) This disease causes the death of seedlings and consequently results in economic losses for sugarcane growers. The objective of this work was to isolate a lactic acid bacterium with antimicrobial activity against X. albilineans from sugarcane crops and to evaluate its antimicrobial activity and its lactic acid production kinetics, biomass yield, and substrate consumption in three different fermentation substrates. To isolate the lactic acid bacterium, samples were collected from different parts of infected and non-infected sugarcane plants of var. CC85-92. Lactococcus lactis check for this species in other resources ssp. lactis was isolated from the leaves of healthy crops, and showed in vitro antimicrobial activity against the pathogen. Batch fermentations of this isolate (at 32 °C, agitation of 100 rpm, and pH 6) were performed using a commercial substrate (MRS), a commercial substrate supplemented with glucose (MRSG), and a substrate produced from agricultural crop residues (ACR). The highest antimicrobial activity was 5.83 mm in the ACR substrate after 6 h of fermentation. The maximum biomass production of 3.37 g L-1 and the maximum lactic acid production of 12.1 g L-1 were obtained in the MRSG substrate. The lactic acid production did not show any significant differences between the substrates. This lactic acid bacterium showed antimicrobial activity against X. albilineans and is thus a biological alternative for the control of leaf scald disease in sugarcane.

Keywords
ACR; Lactococcus lactis ssp. lactis; leaf scald; Saccharum officinarum

 
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