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Chilean Journal of Agricultural Research
Instituto de Investigaciones Agropecuarias, INIA
ISSN: 0718-5820
EISSN: 0718-5839
Vol. 73, No. 4, 2013, pp. 372-376
Bioline Code: cj13054
Full paper language: English
Document type: Research Article
Document available free of charge

Chilean Journal of Agricultural Research, Vol. 73, No. 4, 2013, pp. 372-376

 en Isoflavone content in red clover ( Trifolium pratense check for this species in other resources L.) as related to nitrogen and phosphorus application rate
Du, Wenhua; Tian, Xinhui; Yue, Yan & Lu, Juan


Red clover ( Trifolium pratense check for this species in other resources L.) as a dietary supplement is currently used to treat menopausal symptoms because of its high concentrations of mildly estrogenic isoflavone. The application rate of N and P fertilizer has a huge influence on the isoflavone content (IC) in red clover. Trifolium pratense ‘Minshan’ is a unique plant resource in the alpine pasture region of northwest China. It plays a very important role in livestock production and the pharmaceutical industry because of its high nutritional value and IC. To date, little information exists on the selection of optimal N and P application rates to maximize the IC in red clover. Therefore, a 3-yr study was conducted to define the optimum N and P application rate to achieve a high IC in ‘Minshan’. The results showed that topdressing 50 kg N ha-1 yr-1 with 52-105 kg P ha-1 yr-1 could produce a high level of IC at the branching, flower bud, early flowering, full flowering, pod setting, and mature stages in red clover. Second-year crops produced higher IC than did third-year crops. The IC was highest during the vegetative growing period and started to decline following the reproductive development of the plant until the seeds matured.

Fertilization rate; growth stage; isoflavone content; Minshan red clover

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