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Chilean Journal of Agricultural Research
Instituto de Investigaciones Agropecuarias, INIA
ISSN: 0718-5820
EISSN: 0718-5839
Vol. 74, No. 3, 2014, pp. 273-279
Bioline Code: cj14040
Full paper language: English
Document type: Research Article
Document available free of charge

Chilean Journal of Agricultural Research, Vol. 74, No. 3, 2014, pp. 273-279

 en Essential oils as biological alternatives to protect date palm ( Phoenix dactylifera check for this species in other resources L.) against Ectomyelois ceratoniae Zeller (Lepidoptera: Pyralidae)
Amri, Ismail; Hamrouni, Lamia; Hanana, Mohsen; Jamoussi, Bassem & Lebdi, Kaouthar


The aims of this research were to determine the chemical composition of the essential oil of three Tunisian plants and to evaluate their biological activity against eggs, larvae, and adult insects of Ectomyelois ceratoniae Zeller. The essential oils extracted from leaves of Thymus capitatus check for this species in other resources (L.) Hoffmanns. & Link, Rosmarinus officinalis check for this species in other resources L. and needles of Pinus halepensis check for this species in other resources Mill. were analyzed by gas chromatography-mass spectrometry; 34, 16, and 56 constituents were identified, respectively. The major constituents were (Z)-caryophyllene (23.8%), β-myrcene (20.5%) and α-pinene (13.3%) in P. halepensis oil, carvacrol (66.9%), p-cymene (9.1%), and δ-terpinene (6.2%) in T. capitatus oil and 1,8-cineole (47.5%), camphor (14.9%), α-pinene (14.1%), and borneol (13.1%) in R. officinalis oil. The insecticidal effects of essential oils on eggs, larvae, and adults of E. ceratoniae were investigated. Ovicidal activity of oils was studied by spray on eggs while larvicidal and adulticidal activities were assessed by fumigation and spray. Number of hatched eggs was verified after 10 d, larva and adult mortalities were observed after 6, 12, and 24 h. Globally, eggs and larvae were the most resistant to the three different oils, needing higher doses to obtain a higher mortality. The spray method was most effective than fumigation. Essential oil extracted from T. capitatus proved to be very toxic towards E. ceratoniae on all three phases at the dose of 20 μL mL-1 (100% inhibition), followed by the oil from R. officinalis (90-100% inhibition), nevertheless, weak activity was obtained with P. halepensis oil (68.3-85% inhibition). Results obtained may suggest that the essential oils of T. capitatus and R. officinalis possess high insecticidal activity and therefore, can be used in biotechnological application as natural preservative in stored dates and could be useful in managing populations of E. ceratoniae in field.

Bio-pesticides; Ectomyelois ceratoniae; essential oils; fumigation; insecticidal activity

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