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Chilean Journal of Agricultural Research
Instituto de Investigaciones Agropecuarias, INIA
ISSN: 0718-5820
EISSN: 0718-5839
Vol. 74, No. 4, 2014, pp. 427-431
Bioline Code: cj14061
Full paper language: English
Document type: Research Article
Document available free of charge

Chilean Journal of Agricultural Research, Vol. 74, No. 4, 2014, pp. 427-431

 en Complete control of Penicillium expansum check for this species in other resources on apple fruit using a combination of antagonistic yeast Candida oleophila check for this species in other resources
Guerrero, Víctor; Guigon, Cesar; Berlanga, David & Ojeda, Damaris

Abstract

Three Candida oleophila check for this species in other resources strains (L06, L07 smooth, and L07 rough) were evaluated in vivo and in vitro as biocontrol agents against Penicillium expansum check for this species in other resources on postharvest ‘Golden Delicious’ apples ( Malus domestica check for this species in other resources Borkh.) in Chihuahua, Mexico. The in vivo and in vitro activity of exo-β-1,3-glucanase was measured as a possible biocontrol mode of action for C. oleophila. Mean disease incidence caused by P. expansum was 0.3% for apples treated with fludioxonil + ciprodinil, which were used as a positive control, and 1% for fruits treated with a combination of the three C. oleophila strains; the effects of these treatments were significantly equivalent. Disease incidence in control apples was 39% and was significantly different from the other treatments. The in vivo exo-β-1,3-glucanase activity began at 24 h and peaked at 72 and 96 h for all treatments. Strain L06 had the highest activity (7.96 nKat) and a specific activity of 2.92 nKat μg-1. Candida albicans check for this species in other resources had the lowest activity (2.83 nKat) and a specific activity of 0.67 nKat μg-1. The highest in vitro activity was for C. albicans (85.03 nKat) and the lowest for strain L06 (78.2 nKat). Significant differences in both in vivo and in vitro enzymatic activity were observed between strain L06 and C. albicans. Polynomial regression analysis (R2 = 0.96 in vitro and 0.68 in vivo) indicated that increased enzymatic activity was associated with reduced fruit disease incidence. The production of exo-β-1,3-glucanase by C. oleophila is a possible mode of action for the efficient biocontrol of P. expansum on postharvest apples.

Keywords
Biocontrol; Malus domestica; yeasts

 
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