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Chilean Journal of Agricultural Research
Instituto de Investigaciones Agropecuarias, INIA
ISSN: 0718-5820
EISSN: 0718-5839
Vol. 75, No. 3, 2015, pp. 298-306
Bioline Code: cj15040
Full paper language: English
Document type: Research Article
Document available free of charge

Chilean Journal of Agricultural Research, Vol. 75, No. 3, 2015, pp. 298-306

 en Different aspects of Lactobacillus check for this species in other resources inoculants on the improvement of quality and safety of alfalfa silage
Zielińska, Krystyna; Fabiszewska, Agata & Stefańska, Ilona

Abstract

There is a significant range of bacterial inoculants for forage ensiling, but there is still a need for formulations to improve the safety of feed. The objective of the study was to evaluate the usefulness of three lactobacilli strains in alfalfa ( Medicago sativa check for this species in other resources L.) The following bacterial inoculants were used: Lactocacillus plantarum check for this species in other resources K KKP 593p (LPK), L. plantarum C KKP 788p (LPC), L. buchneri check for this species in other resources KKP 907p (LB), and mix of all three strains (LPK+LPC+LB). The application of bacterial inoculants in alfalfa ensiling resulted in a reduction of the total number of molds, Clostridium perfringens check for this species in other resources and Listeria check for this species in other resources sp. (up to 5, 7, and 5 times respectively for LB inoculant in comparison to untreated silage). Total inhibition of Salmonella check for this species in other resources sp. and Escherichia coli check for this species in other resources growth was achieved in silages treated with all inoculants except for LPC. Aerobic stability in the control silage was the lowest (77 h) and doubled under the influence of bacterial inoculants. The most stable according to aerobic stability was silage treated with LB inoculant (175 h), where the highest concentrations of acetic acid (4.8 g kg-1), propionic acid (0.7 g kg-1) and 1,2-propanediol (526 mg kg-1) were reported. The study discussed that it is important to evaluate not only the effect of bacterial inoculants on physicochemical and microbiological silage properties, as the presence and expression of antibiotic resistance genes in lactic acid bacteria have been reported. The results of antimicrobial susceptibility testing of the strains showed that almost all minimum inhibitory concentrations values for eight antibiotics were equal to or below the corresponding breakpoints proposed by the European Food Safety Authority, Additives and Products or Substances used in Animal Feed Panel.

Keywords
Aerobic stability; alfalfa; antibiotic susceptibility; Lactobacillus; pathogenic bacteria; silage

 
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