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Chilean Journal of Agricultural Research
Instituto de Investigaciones Agropecuarias, INIA
ISSN: 0718-5820
EISSN: 0718-5839
Vol. 75, No. 4, 2015, pp. 488-496
Bioline Code: cj15065
Full paper language: English
Document type: Research Article
Document available free of charge

Chilean Journal of Agricultural Research, Vol. 75, No. 4, 2015, pp. 488-496

 en Effect of chemical fertilization and green manure on the abundance and community structure of ammonia oxidizers in a paddy soil
Fang, Yu; Yan, Zhi-Lei; Chen, Ji-Chen; Wang, Fei; Wang, Ming-Kuang & Lin, Xin-Jian

Abstract

Ammonia oxidization is a critical step in the soil N cycle and can be affected by the fertilization regimes. Chinese milkvetch ( Astragalus sinicus check for this species in other resources L., MV) is a major green manure of rice ( Oryza sativa check for this species in other resources L.) fields in southern China, which is recommended as an important agronomic practice to improve soil fertility. Soil chemical properties, abundance and community structures of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) in a MV-rice rotation field under different fertilization regimes were investigated. The field experiment included six treatments: control, without MV and chemical fertilizer (CK); 100% chemical fertilizer (NPK); 18 000 kg MV ha-1 plus 100% chemical fertilizer (NPKM1); 18 000 kg MV ha-1 plus 40% chemical fertilizer (NPKM2); 18 000 kg MV ha-1 alone (MV); and 18 000 kg MV ha-1 plus 40% chemical fertilizer plus straw (NPKMS). Results showed that NPKMS treatment could improve the soil fertility greatly although the application of 60% chemical fertilizer. The abundance of AOB only in the MV treatment had significant difference with the control; AOA were more abundant than AOB in all corresponding treatments. The NPKMS treatment had the highest AOA abundance (1.19 × 108 amoA gene copies g-1) and the lowest abundance was recorded in the CK treatment (3.21 × 107 amoA gene copies g-1). The abundance of AOA was significantly positively related to total N, available N, NH4+-N, and NO3--N. The community structure of AOA exhibited little variation among different fertilization regimes, whereas the community structure of AOB was highly responsive. Phylogenetic analysis showed that all AOB sequences were affiliated with Nitrosospira check for this species in other resources or Nitrosomonas check for this species in other resources and all AOA denaturing gradient gel electrophoresis (DGGE) bands belonged to the soil and sediment lineage. These findings could be fundamental to improve our understanding of AOB and AOA in the N cycle in the paddy soil.

Keywords
Abundance; ammonia-oxidizing archaea (AOA); ammonia-oxidizing bacteria (AOB); Astragalus sinicus; community structure; Chinese milk vetch; soil chemical properties

 
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